Values CatLog read from this paper
Altering substrate specificity of Bacillus sp. SAM1606 alpha-glucosidase by comparative site-specific mutagenesis
PMID 8999834doi:10.1074/jbc.272.3.1601Open in Browse
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CatLog read 42 records (42 values) from this paper: alpha-glucosidase from Bacillus sp. SAM1606.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alpha-glucosidase | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 9.3 | — | pH 6, 55 °C | Open record: alpha-glucosidase, alpha,alpha'-trehalose |
| alpha-glucosidase | Bacillus sp. SAM1606 | isomaltose | — | 3.5 | — | pH 6, 55 °C | Open record: alpha-glucosidase, isomaltose |
| alpha-glucosidase | Bacillus sp. SAM1606 | maltose | — | 7.5 | — | pH 6, 55 °C | Open record: alpha-glucosidase, maltose |
| alpha-glucosidase | Bacillus sp. SAM1606 | sucrose | — | 6.9 | — | pH 6, 55 °C | Open record: alpha-glucosidase, sucrose |
| alpha-glucosidasevariant A116V | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 6.8 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant A116V, alpha,alpha'-trehalose |
| alpha-glucosidasevariant A116V | Bacillus sp. SAM1606 | isomaltose | — | 1.7 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant A116V, isomaltose |
| alpha-glucosidasevariant A116V | Bacillus sp. SAM1606 | maltose | — | 13.9 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant A116V, maltose |
| alpha-glucosidasevariant A116V | Bacillus sp. SAM1606 | sucrose | — | 8.5 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant A116V, sucrose |
| alpha-glucosidasevariant G273P | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 113 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant G273P, alpha,alpha'-trehalose |
| alpha-glucosidasevariant G273P | Bacillus sp. SAM1606 | isomaltose | — | 5.8 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant G273P, isomaltose |
| alpha-glucosidasevariant G273P | Bacillus sp. SAM1606 | maltose | — | 11.5 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant G273P, maltose |
| alpha-glucosidasevariant G273P | Bacillus sp. SAM1606 | sucrose | — | 9.1 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant G273P, sucrose |
| alpha-glucosidasevariant G273P/T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 88.7 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant G273P/T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant G273P/T342N | Bacillus sp. SAM1606 | isomaltose | — | 1.4 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant G273P/T342N, isomaltose |
| alpha-glucosidasevariant M76N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 4.7 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant M76N | Bacillus sp. SAM1606 | isomaltose | — | 4 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N, isomaltose |
| alpha-glucosidasevariant M76N | Bacillus sp. SAM1606 | maltose | — | 4.4 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N, maltose |
| alpha-glucosidasevariant M76N | Bacillus sp. SAM1606 | sucrose | — | 7.2 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N, sucrose |
| alpha-glucosidasevariant M76N/A116V/G273P/T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 1001 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/A116V/G273P/T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant M76N/A116V/G273P/T342N | Bacillus sp. SAM1606 | isomaltose | — | 16 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/A116V/G273P/T342N, isomaltose |
| alpha-glucosidasevariant M76N/R81S/A116V/G273P | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 117 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/G273P, alpha,alpha'-trehalose |
| alpha-glucosidasevariant M76N/R81S/A116V/G273P | Bacillus sp. SAM1606 | isomaltose | — | 16.1 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/G273P, isomaltose |
| alpha-glucosidasevariant M76N/R81S/A116V/G273P/T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 1183 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/G273P/T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant M76N/R81S/A116V/G273P/T342N | Bacillus sp. SAM1606 | isomaltose | — | 7.6 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/G273P/T342N, isomaltose |
| alpha-glucosidasevariant M76N/R81S/A116V/G273P/T342N | Bacillus sp. SAM1606 | maltose | — | 36.2 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/G273P/T342N, maltose |
| alpha-glucosidasevariant M76N/R81S/A116V/G273P/T342N | Bacillus sp. SAM1606 | sucrose | — | 10.5 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/G273P/T342N, sucrose |
| alpha-glucosidasevariant M76N/R81S/A116V/T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 4.9 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant M76N/R81S/A116V/T342N | Bacillus sp. SAM1606 | isomaltose | — | 9.9 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/T342N, isomaltose |
| alpha-glucosidasevariant M76N/R81S/A116V/T342N | Bacillus sp. SAM1606 | maltose | — | 1.9 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/T342N, maltose |
| alpha-glucosidasevariant M76N/R81S/A116V/T342N | Bacillus sp. SAM1606 | sucrose | — | 16.4 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/A116V/T342N, sucrose |
| alpha-glucosidasevariant M76N/R81S/G273P/T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 1.21 × 10³ | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/G273P/T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant M76N/R81S/G273P/T342N | Bacillus sp. SAM1606 | isomaltose | — | 13.9 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant M76N/R81S/G273P/T342N, isomaltose |
| alpha-glucosidasevariant R81S | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 12.1 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant R81S, alpha,alpha'-trehalose |
| alpha-glucosidasevariant R81S | Bacillus sp. SAM1606 | isomaltose | — | 3.7 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant R81S, isomaltose |
| alpha-glucosidasevariant R81S | Bacillus sp. SAM1606 | maltose | — | 5.8 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant R81S, maltose |
| alpha-glucosidasevariant R81S | Bacillus sp. SAM1606 | sucrose | — | 7.2 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant R81S, sucrose |
| alpha-glucosidasevariant R81S/A116V/G273P/T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 1103 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant R81S/A116V/G273P/T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant R81S/A116V/G273P/T342N | Bacillus sp. SAM1606 | isomaltose | — | 9 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant R81S/A116V/G273P/T342N, isomaltose |
| alpha-glucosidasevariant T342N | Bacillus sp. SAM1606 | alpha,alpha'-trehalose | — | 9.8 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant T342N, alpha,alpha'-trehalose |
| alpha-glucosidasevariant T342N | Bacillus sp. SAM1606 | isomaltose | — | 3.8 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant T342N, isomaltose |
| alpha-glucosidasevariant T342N | Bacillus sp. SAM1606 | maltose | — | 2.4 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant T342N, maltose |
| alpha-glucosidasevariant T342N | Bacillus sp. SAM1606 | sucrose | — | 6.8 | — | pH 6, 55 °C | Open record: alpha-glucosidase variant T342N, sucrose |