CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of a hydrophobic exosite on tissue type plasminogen activator that modulates specificity for plasminogen

PMID 8999865doi:10.1074/jbc.272.3.1811Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 60 records (180 values) from this paper: tissue type plasminogen activator (t-PA) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.68

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
tissue type plasminogen activator (t-PA)Homo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.11.1 × 10⁻⁵9.1 × 10³Open
tissue type plasminogen activator (t-PA)Homo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0680.00514Open
tissue type plasminogen activator (t-PA)Homo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline470.4120Open
tissue type plasminogen activator (t-PA)variant F423AHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.121.4 × 10⁻⁵8.6 × 10³Open
tissue type plasminogen activator (t-PA)variant F423AHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.020.0045Open
tissue type plasminogen activator (t-PA)variant F423AHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline490.598Open
tissue type plasminogen activator (t-PA)variant F423A/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.22.2 × 10⁻⁵9.1 × 10³Open
tissue type plasminogen activator (t-PA)variant F423A/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0040.010.4Open
tissue type plasminogen activator (t-PA)variant F423A/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline27127Open
tissue type plasminogen activator (t-PA)variant F423EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.182.3 × 10⁻⁵7.8 × 10³Open
tissue type plasminogen activator (t-PA)variant F423EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0170.0053.4Open
tissue type plasminogen activator (t-PA)variant F423EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline570.5110Open
tissue type plasminogen activator (t-PA)variant F423E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.151.5 × 10⁻⁵1 × 10⁴Open
tissue type plasminogen activator (t-PA)variant F423E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0030.0080.38Open
tissue type plasminogen activator (t-PA)variant F423E/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline280.931Open
tissue type plasminogen activator (t-PA)variant L420AHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.171.9 × 10⁻⁵8.9 × 10³Open
tissue type plasminogen activator (t-PA)variant L420AHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0140.00114Open
tissue type plasminogen activator (t-PA)variant L420AHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline450.4110Open
tissue type plasminogen activator (t-PA)variant L420A/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.181.4 × 10⁻⁵1.3 × 10⁴Open
tissue type plasminogen activator (t-PA)variant L420A/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0080.0090.89Open
tissue type plasminogen activator (t-PA)variant L420A/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline421.332Open
tissue type plasminogen activator (t-PA)variant L420EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.172.1 × 10⁻⁵8.1 × 10³Open
tissue type plasminogen activator (t-PA)variant L420EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0057 × 10⁻⁴7.1Open
tissue type plasminogen activator (t-PA)variant L420EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline450.4110Open
tissue type plasminogen activator (t-PA)variant L420E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.151.8 × 10⁻⁵8.3 × 10³Open
tissue type plasminogen activator (t-PA)variant L420E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0020.0050.4Open
tissue type plasminogen activator (t-PA)variant L420E/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline290.932Open
tissue type plasminogen activator (t-PA)variant P422AHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.111.3 × 10⁻⁵8.5 × 10³Open
tissue type plasminogen activator (t-PA)variant P422AHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0280.0047Open
tissue type plasminogen activator (t-PA)variant P422AHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline470.4120Open
tissue type plasminogen activator (t-PA)variant P422A/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.151 × 10⁻⁵1.5 × 10⁴Open
tissue type plasminogen activator (t-PA)variant P422A/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0070.0130.54Open
tissue type plasminogen activator (t-PA)variant P422A/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline321.227Open
tissue type plasminogen activator (t-PA)variant P422EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.152.9 × 10⁻⁵5.2 × 10³Open
tissue type plasminogen activator (t-PA)variant P422EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.00157 × 10⁻⁴2.1Open
tissue type plasminogen activator (t-PA)variant P422EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline490.4120Open
tissue type plasminogen activator (t-PA)variant P422E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.172.8 × 10⁻⁵6.1 × 10³Open
tissue type plasminogen activator (t-PA)variant P422E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM5 × 10⁻⁴0.0050.1Open
tissue type plasminogen activator (t-PA)variant P422E/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline311.128Open
tissue type plasminogen activator (t-PA)variant P422GHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.152.6 × 10⁻⁵5.8 × 10³Open
tissue type plasminogen activator (t-PA)variant P422GHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.00286 × 10⁻⁴4.7Open
tissue type plasminogen activator (t-PA)variant P422GHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline600.6100Open
tissue type plasminogen activator (t-PA)variant P422G/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.212.7 × 10⁻⁵7.8 × 10³Open
tissue type plasminogen activator (t-PA)variant P422G/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM8 × 10⁻⁴0.0040.2Open
tissue type plasminogen activator (t-PA)variant P422G/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline141.78.2Open
tissue type plasminogen activator (t-PA)variant R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.131 × 10⁻⁵1.3 × 10⁴Open
tissue type plasminogen activator (t-PA)variant R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0240.0171.4Open
tissue type plasminogen activator (t-PA)variant R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline33133Open
tissue type plasminogen activator (t-PA)variant S421EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.151.8 × 10⁻⁵8.3 × 10³Open
tissue type plasminogen activator (t-PA)variant S421EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0160.0035.3Open
tissue type plasminogen activator (t-PA)variant S421EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline460.4120Open
tissue type plasminogen activator (t-PA)variant S421E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.212.3 × 10⁻⁵9.1 × 10³Open
tissue type plasminogen activator (t-PA)variant S421E/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0020.0070.29Open
tissue type plasminogen activator (t-PA)variant S421E/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline262.311Open
tissue type plasminogen activator (t-PA)variant S421GHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.111.6 × 10⁻⁵6.9 × 10³Open
tissue type plasminogen activator (t-PA)variant S421GHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0180.0044.5Open
tissue type plasminogen activator (t-PA)variant S421GHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline360.490Open
tissue type plasminogen activator (t-PA)variant S421G/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.0125-0.2 µM0.192.6 × 10⁻⁵7.3 × 10³Open
tissue type plasminogen activator (t-PA)variant S421G/R275EHomo sapiensLys-plasminogenLys-plasminogen varied 0.9-15 µM0.0010.0040.25Open
tissue type plasminogen activator (t-PA)variant S421G/R275EHomo sapiensmethylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline234.65Open