EC 3.4.21.68: t-Plasminogen activator

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CatLog read 194 records for EC 3.4.21.68 from 6 papers, for 2 organisms. Names in these papers: tissue type plasminogen activator (t-PA), tissue-type plasminogen activator (tPA), tissue-type plasminogen activator (single-chain tPA), tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA) and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 9 records for EC 3.4.21.68 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.68 in Browse.

Papers

Papers with records for EC 3.4.21.68
Paper	Organisms	Records
Analysis of tissue plasminogen activator specificity using peptidyl fluorogenic substrates	—	76
Identification of a hydrophobic exosite on tissue type plasminogen activator that modulates specificity for plasminogen	Homo sapiens	60
Structural mapping of the active site specificity determinants of human tissue-type plasminogen activator. Implications for the design of low molecular weight substrates and inhibitors	Homo sapiens	29
The regulation by fibrinogen and fibrin of tissue plasminogen activator kinetics and inhibition by plasminogen activator inhibitor 1	—	12
Directing sequence-specific proteolysis to new targets. The influence of loop size and target sequence on selective proteolysis by tissue-type plasminogen activator and urokinase-type plasminogen…	—	9
Differences and Compatibility between Human and Porcine Fibrinolytic Components toward Plasmin Generation and Fibrin Degradation	Homo sapiens, Sus scrofa	8

Records

Records for EC 3.4.21.68 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
tissue plasminogen activator (tPA)	—	H-D-Ile-Pro-Arg-pNA	10.7	0.694	15		PMID 17408411	Open record: tissue plasminogen activator (tPA), H-D-Ile-Pro-Arg-pNA
tissue plasminogen activator (tPA)	—	plasminogenplasminogen activation in buffer; no promoter	—	—	27		PMID 17408411	Open record: tissue plasminogen activator (tPA), plasminogen
tissue plasminogen activator (tPA)	—	plasminogen	—	—	140		PMID 17408411	Open record: tissue plasminogen activator (tPA), plasminogen
tissue plasminogen activator (tPA)	—	plasminogenplasminogen activation in fibrin clots; apparent kcat	0.027	1.09 × 10⁻⁴	250		PMID 17408411	Open record: tissue plasminogen activator (tPA), plasminogen
tissue plasminogen activator (tPA)	—	plasminogenplasminogen activation in fibrin clots; apparent kcat	0.026	3.62 × 10⁻⁴	70		PMID 17408411	Open record: tissue plasminogen activator (tPA), plasminogen
tissue plasminogen activator (tPA)	—	plasminogenplasminogen activation in fibrin clots; apparent kcat	0.028	1.19 × 10⁻⁴	240		PMID 17408411	Open record: tissue plasminogen activator (tPA), plasminogen
tissue plasminogen activator (tPA)variant R275E (sctPA)	—	H-D-Ile-Pro-Arg-pNA	7.3	1.46	5		PMID 17408411	Open record: tissue plasminogen activator (tPA) variant R275E (sctPA), H-D-Ile-Pro-Arg-pNA
tissue plasminogen activator (tPA)variant R275E (sctPA)	—	plasminogenplasminogen activation in buffer; no promoter	—	—	4		PMID 17408411	Open record: tissue plasminogen activator (tPA) variant R275E (sctPA), plasminogen
tissue plasminogen activator (tPA)variant R275E (sctPA)	—	plasminogen	—	—	160		PMID 17408411	Open record: tissue plasminogen activator (tPA) variant R275E (sctPA), plasminogen
tissue plasminogen activator (tPA)variant R275E (sctPA)	—	plasminogenplasminogen activation in fibrin clots; apparent kcat	0.023	8.7 × 10⁻⁵	270		PMID 17408411	Open record: tissue plasminogen activator (tPA) variant R275E (sctPA), plasminogen
tissue plasminogen activator (tPA)variant R275E (sctPA)	—	plasminogenplasminogen activation in fibrin clots; apparent kcat	0.024	3.41 × 10⁻⁴	70		PMID 17408411	Open record: tissue plasminogen activator (tPA) variant R275E (sctPA), plasminogen
tissue plasminogen activator (tPA)variant R275E (sctPA)	—	plasminogenplasminogen activation in fibrin clots; apparent kcat	0.023	8.6 × 10⁻⁵	270		PMID 17408411	Open record: tissue plasminogen activator (tPA) variant R275E (sctPA), plasminogen
tissue type plasminogen activator (t-PA)	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.1	1.1 × 10⁻⁵	9.1 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA), Lys-plasminogen
tissue type plasminogen activator (t-PA)	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.068	0.005	14		PMID 8999865	Open record: tissue type plasminogen activator (t-PA), Lys-plasminogen
tissue type plasminogen activator (t-PA)	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	47	0.4	120		PMID 8999865	Open record: tissue type plasminogen activator (t-PA), methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant F423A	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.12	1.4 × 10⁻⁵	8.6 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423A, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423A	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.02	0.004	5		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423A, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423A	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	49	0.5	98		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423A, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant F423A/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.2	2.2 × 10⁻⁵	9.1 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423A/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423A/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.004	0.01	0.4		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423A/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423A/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	27	1	27		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423A/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant F423E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.18	2.3 × 10⁻⁵	7.8 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.017	0.005	3.4		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	57	0.5	110		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant F423E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.15	1.5 × 10⁻⁵	1 × 10⁴		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.003	0.008	0.38		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant F423E/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	28	0.9	31		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant F423E/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant L420A	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.17	1.9 × 10⁻⁵	8.9 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420A, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420A	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.014	0.001	14		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420A, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420A	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	45	0.4	110		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420A, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant L420A/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.18	1.4 × 10⁻⁵	1.3 × 10⁴		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420A/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420A/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.008	0.009	0.89		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420A/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420A/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	42	1.3	32		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420A/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant L420E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.17	2.1 × 10⁻⁵	8.1 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.005	7 × 10⁻⁴	7.1		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	45	0.4	110		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant L420E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.15	1.8 × 10⁻⁵	8.3 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.002	0.005	0.4		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant L420E/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	29	0.9	32		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant L420E/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant P422A	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.11	1.3 × 10⁻⁵	8.5 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422A, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422A	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.028	0.004	7		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422A, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422A	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	47	0.4	120		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422A, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant P422A/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.15	1 × 10⁻⁵	1.5 × 10⁴		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422A/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422A/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.007	0.013	0.54		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422A/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422A/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	32	1.2	27		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422A/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant P422E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.15	2.9 × 10⁻⁵	5.2 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.0015	7 × 10⁻⁴	2.1		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	49	0.4	120		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant P422E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.17	2.8 × 10⁻⁵	6.1 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	5 × 10⁻⁴	0.005	0.1		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422E/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	31	1.1	28		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422E/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant P422G	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.15	2.6 × 10⁻⁵	5.8 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422G, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422G	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.0028	6 × 10⁻⁴	4.7		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422G, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422G	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	60	0.6	100		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422G, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant P422G/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.21	2.7 × 10⁻⁵	7.8 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422G/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422G/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	8 × 10⁻⁴	0.004	0.2		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422G/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant P422G/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	14	1.7	8.2		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant P422G/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.13	1 × 10⁻⁵	1.3 × 10⁴		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.024	0.017	1.4		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	33	1	33		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant S421E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.15	1.8 × 10⁻⁵	8.3 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.016	0.003	5.3		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	46	0.4	120		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant S421E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.21	2.3 × 10⁻⁵	9.1 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421E/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.002	0.007	0.29		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421E/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421E/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	26	2.3	11		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421E/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant S421G	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.11	1.6 × 10⁻⁵	6.9 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421G, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421G	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.018	0.004	4.5		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421G, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421G	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	36	0.4	90		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421G, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue type plasminogen activator (t-PA)variant S421G/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.0125-0.2 µM	0.19	2.6 × 10⁻⁵	7.3 × 10³		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421G/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421G/R275E	Homo sapiens	Lys-plasminogenLys-plasminogen varied 0.9-15 µM	0.001	0.004	0.25		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421G/R275E, Lys-plasminogen
tissue type plasminogen activator (t-PA)variant S421G/R275E	Homo sapiens	methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline	23	4.6	5		PMID 8999865	Open record: tissue type plasminogen activator (t-PA) variant S421G/R275E, methylsulfonyl-D-cyclohexyltyrosyl-glycyl-arginine-p-nitroaniline
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	D-alpha-aspartyl-glycyl-L-arginine 4-nitroanilide	0.43	1.7	0.25	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), D-alpha-aspartyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	D-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide	3.98	2.9	1.37	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), D-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	D-cyclohexylglycyl-glycyl-L-arginine 4-nitroanilide	6.74	1.53	4.4	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), D-cyclohexylglycyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	D-hexahydrotyrosyl-glycyl-L-arginine 4-nitroanilide	10.6	0.83	12.8	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), D-hexahydrotyrosyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	D-lysyl-glycyl-L-arginine 4-nitroanilide	6.32	2.1	3.01	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), D-lysyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	D-phenylalanyl-glycyl-L-arginine 4-nitroanilide	7.46	0.7	10.7	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), D-phenylalanyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	L-alpha-aspartyl-glycyl-L-arginine 4-nitroanilide	0.28	0.86	0.32	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), L-alpha-aspartyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	L-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide	0.13	3.2	0.041	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), L-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	L-cyclohexylglycyl-glycyl-L-arginine 4-nitroanilide	1.36	5.32	0.26	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), L-cyclohexylglycyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	L-phenylalanyl-glycyl-L-arginine 4-nitroanilide	2.79	2	1.4	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), L-phenylalanyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-D-alpha-aspartyl(beta-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide	4.98	1.8	2.77	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-D-alpha-aspartyl(beta-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-D-alpha-glutamyl(gamma-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide	4.48	1.2	3.73	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-D-alpha-glutamyl(gamma-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-D-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide	7.46	2.6	2.87	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-D-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine 4-nitroanilide	8.53	0.52	16.4	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-D-phenylalanyl-glycyl-L-arginine 4-nitroanilide	8.96	0.3	19.9	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-D-phenylalanyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-L-alpha-aspartyl(beta-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide	1.84	2.3	0.8	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-L-alpha-aspartyl(beta-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-L-alpha-aspartyl-glycyl-L-arginine 4-nitroanilide	0.35	0.72	0.49	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-L-alpha-aspartyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-L-alpha-glutamyl(gamma-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide	1.29	0.9	1.43	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-L-alpha-glutamyl(gamma-tert-butyl ester)-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-L-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide	0.75	2.2	0.34	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-L-alpha-glutamyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-benzyloxycarbonyl-L-valyl-glycyl-L-arginine 4-nitroanilide	1.69	1.36	1.24	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-benzyloxycarbonyl-L-valyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methoxycarbonyl-D-cyclohexylalanyl-glycyl-L-arginine 4-nitroanilide	7.76	0.34	22.8	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methoxycarbonyl-D-cyclohexylalanyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-arginine 4-nitroanilide	5.02	0.42	11.9	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methoxycarbonyl-D-hexahydrotyrosyl-glycyl-L-arginine 4-nitroanilide	8.31	0.39	21.3	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methoxycarbonyl-D-hexahydrotyrosyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methoxycarbonyl-D-leucyl-glycyl-L-arginine 4-nitroanilide	6.92	0.62	11.2	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methoxycarbonyl-D-leucyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methoxycarbonyl-D-norleucyl-glycyl-L-arginine 4-nitroanilide	6.82	0.99	6.89	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methoxycarbonyl-D-norleucyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methoxycarbonyl-D-valyl-glycyl-L-arginine 4-nitroanilide	5.77	0.8	7.22	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methoxycarbonyl-D-valyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-methylsulfonyl-D-phenylalanyl-glycyl-L-arginine 4-nitroanilide	7.36	0.1	73.6	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-methylsulfonyl-D-phenylalanyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	N-tert-butoxycarbonyl-glycyl-glycyl-L-arginine 4-nitroanilide	1.29	3.5	0.37	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), N-tert-butoxycarbonyl-glycyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (single-chain tPA)	Homo sapiens	pyroglutamyl-glycyl-L-arginine 4-nitroanilide	4.04	3.15	1.28	pH 8, 25 °C	PMID 9268299	Open record: tissue-type plasminogen activator (single-chain tPA), pyroglutamyl-glycyl-L-arginine 4-nitroanilide
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA	—	—	0.029	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (II), residues 44-51 of the 43-52 loop replaced by PFGRSAG	0.018	0.079	0.23	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (II), residues 44-51 of the 43-52 loop replaced by PFGRSAG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (III), residues 44-51 of the 43-52 loop replaced by PPFGRSAG	0.011	0.0099	1.1	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (III), residues 44-51 of the 43-52 loop replaced by PPFGRSAG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (IV), residues 44-51 of the 43-52 loop replaced by PGPFGRSAG	0.0075	0.0099	0.76	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (IV), residues 44-51 of the 43-52 loop replaced by PGPFGRSAG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (IX), residues 44-51 of the 43-52 loop replaced by PGSGRSASGTTGTG	—	—	0.019	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (IX), residues 44-51 of the 43-52 loop replaced by PGSGRSASGTTGTG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG	0.01	0.0093	1.1	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (VI), residues 44-51 of the 43-52 loop replaced by PHYGRSGG	0.012	0.018	0.67	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (VI), residues 44-51 of the 43-52 loop replaced by PHYGRSGG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (VII), residues 44-51 of the 43-52 loop replaced by PQRGRSAG	0.006	0.0089	0.67	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (VII), residues 44-51 of the 43-52 loop replaced by PQRGRSAG
tissue-type plasminogen activator (t-PA)	—	staphylococcal nuclease variant (VIII), residues 44-51 of the 43-52 loop replaced by PGSGRSAG	3.7 × 10⁻⁴	0.017	0.022	37 °C	PMID 9468480	Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (VIII), residues 44-51 of the 43-52 loop replaced by PGSGRSAG
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	1.47	0.39	3.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), (p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.057	0.041	1.4	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.31	0.098	3.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.089	0.069	1.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.021	0.0074	2.9	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.074	0.036	2.1	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.044	0.016	2.7	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.082	0.058	1.4	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.71	0.11	6.4	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	L-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.04	0.97	0.04	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), L-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	L-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.08	0.46	0.18	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), L-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.097	0.015	6.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.54	0.066	8.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.53	0.36	1.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.1	0.03	3.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.15	0.047	3.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.49	0.091	5.4	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.34	0.14	2.4	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.25	0.026	9.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-L-(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	1.03	0.071	14.6	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-L-(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-L-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.011	0.047	0.24	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-L-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA)	—	tert-butyloxycarbonyl-L-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.084	0.1	0.83	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant sc-tPA/tc-tPA mixture (80% sc-tPA, 20% tc-tPA), tert-butyloxycarbonyl-L-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.37	0.011	32.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), (p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.034	0.011	3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.17	0.012	14.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.1	0.017	6.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.018	0.01	1.9	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-diethyl (Table 4: D-FPR-ANSNR1R2 with R1 = C2H5, R2 = C2H5)	0.14	0.12	1.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-diethyl (Table 4: D-FPR-ANSNR1R2 with R1 = C2H5, R2 = C2H5)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-hexamethylene (R1R2 = (CH2)6 ring) (Table 4: D-FPR-ANSNR1R2 with R1R2 = (CH2)6)	0.22	0.096	2.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-hexamethylene (R1R2 = (CH2)6 ring) (Table 4: D-FPR-ANSNR1R2 with R1R2 = (CH2)6)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(2-methoxyethyl) (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C2H4OCH3)	0.66	0.14	4.7	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(2-methoxyethyl) (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C2H4OCH3)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(methoxycarbonylmethyl), i.e. glycine methyl ester (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = CH2COOCH3)	0.15	0.029	5.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(methoxycarbonylmethyl), i.e. glycine methyl ester (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = CH2COOCH3)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-benzyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C6H5CH2)	0.54	0.025	21.6	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-benzyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C6H5CH2)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.51	0.077	6.6	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = c-C6H11)	0.32	0.088	3.6	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = c-C6H11)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-isopropyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = i-C3H7)	0.2	0.074	2.7	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-isopropyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = i-C3H7)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C4H9)	0.47	0.048	9.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C4H9)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-hexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C6H13)	3.6	0.095	37.9	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-hexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C6H13)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-tert-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = t-C4H9)	0.94	0.084	11.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-tert-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = t-C4H9)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.025	0.0078	3.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.19	0.033	5.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.16	0.0081	20.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.04	0.0085	4.7	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.2	0.0088	23.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.097	0.013	7.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.047	0.013	3.6	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.017	0.0026	6.4	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.15	0.032	4.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.18	0.014	12.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.25	0.015	16.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA)variant two-chain tPA (tc-tPA)	—	tert-butyloxycarbonyl-L-(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.44	0.0066	66.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) variant two-chain tPA (tc-tPA), tert-butyloxycarbonyl-L-(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA)	Homo sapiens	human plasminogenporcine	—	—	190	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), human plasminogen
tissue-type plasminogen activator (tPA)	Homo sapiens	human plasminogenhuman	—	—	440	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), human plasminogen
tissue-type plasminogen activator (tPA)	Homo sapiens	porcine plasminogenporcine	—	—	3.3	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), porcine plasminogen
tissue-type plasminogen activator (tPA)	Homo sapiens	porcine plasminogenhuman	—	—	33	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), porcine plasminogen
tissue-type plasminogen activator (tPA)	Sus scrofa	human plasminogenporcine	—	—	26	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), human plasminogen
tissue-type plasminogen activator (tPA)	Sus scrofa	human plasminogenhuman	—	—	880	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), human plasminogen
tissue-type plasminogen activator (tPA)	Sus scrofa	porcine plasminogenporcine	—	—	18	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), porcine plasminogen
tissue-type plasminogen activator (tPA)	Sus scrofa	porcine plasminogenhuman	—	—	36	pH 7.4, 37 °C	PMID 41567719	Open record: tissue-type plasminogen activator (tPA), porcine plasminogen
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.099	0.026	3.7	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), (p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.0084	0.011	0.71	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.061	0.04	1.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.019	0.014	1.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-diethyl (Table 4: D-FPR-ANSNR1R2 with R1 = C2H5, R2 = C2H5)	0.09	0.32	0.28	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-diethyl (Table 4: D-FPR-ANSNR1R2 with R1 = C2H5, R2 = C2H5)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-hexamethylene (R1R2 = (CH2)6 ring) (Table 4: D-FPR-ANSNR1R2 with R1R2 = (CH2)6)	0.13	0.25	0.52	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N,N-hexamethylene (R1R2 = (CH2)6 ring) (Table 4: D-FPR-ANSNR1R2 with R1R2 = (CH2)6)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(2-methoxyethyl) (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C2H4OCH3)	0.27	0.18	1.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(2-methoxyethyl) (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C2H4OCH3)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(methoxycarbonylmethyl), i.e. glycine methyl ester (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = CH2COOCH3)	0.083	0.063	1.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-(methoxycarbonylmethyl), i.e. glycine methyl ester (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = CH2COOCH3)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-benzyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C6H5CH2)	0.45	0.063	7.1	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-benzyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = C6H5CH2)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.016	0.0057	2.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = c-C6H11)	0.15	0.15	1	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = c-C6H11)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-isopropyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = i-C3H7)	0.29	0.33	0.88	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-isopropyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = i-C3H7)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C4H9)	0.33	0.12	2.8	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C4H9)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-hexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C6H13)	1.1	0.099	11.1	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-n-hexyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = n-C6H13)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-tert-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = t-C4H9)	0.25	0.079	3.2	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-tert-butyl (Table 4: D-FPR-ANSNR1R2 with R1 = H, R2 = t-C4H9)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.011	0.0073	1.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.021	0.022	0.95	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.059	0.025	2.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)	0.012	0.023	0.51	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Leu-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-cyclohexyl (C6H11; c-C6H11 per Table 4)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.038	0.015	2.5	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Leu-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.022	0.02	1.1	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Leu-Ser-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)	0.01	0.017	0.6	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Phe-Leu-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C3H7 (propyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.019	0.012	1.7	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Phe-Val-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.036	0.019	1.9	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Val-Gly-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)	0.065	0.029	2.3	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-D-Val-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-C4H9 (butyl, isomer not stated)
tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA)	—	tert-butyloxycarbonyl-L-(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl	0.11	0.016	7.1	pH 7.4, 25 °C	PMID 9047311	Open record: tissue-type plasminogen activator (tPA) single-chain tPA (sc-tPA), tert-butyloxycarbonyl-L-(p-fluoro)Phe-Pro-Arg 6-amino-1-naphthalenesulfonamide (ANSN) leaving group, sulfonamide N-ethyl