Values CatLog read from this paper
Directing sequence-specific proteolysis to new targets. The influence of loop size and target sequence on selective proteolysis by tissue-type plasminogen activator and urokinase-type plasminogen…
PMID 9468480doi:10.1074/jbc.273.8.4323Open in Browse
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CatLog read 20 records (48 values) from this paper: tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), trypsin from Homo sapiens, Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA | — | — | 0.029 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (II), residues 44-51 of the 43-52 loop replaced by PFGRSAG | 0.018 | 0.079 | 0.23 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (II), residues 44-51 of the 43-52 loop replaced by PFGRSAG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (III), residues 44-51 of the 43-52 loop replaced by PPFGRSAG | 0.011 | 0.0099 | 1.1 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (III), residues 44-51 of the 43-52 loop replaced by PPFGRSAG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (IV), residues 44-51 of the 43-52 loop replaced by PGPFGRSAG | 0.0075 | 0.0099 | 0.76 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (IV), residues 44-51 of the 43-52 loop replaced by PGPFGRSAG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (IX), residues 44-51 of the 43-52 loop replaced by PGSGRSASGTTGTG | — | — | 0.019 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (IX), residues 44-51 of the 43-52 loop replaced by PGSGRSASGTTGTG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG | 0.01 | 0.0093 | 1.1 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (VI), residues 44-51 of the 43-52 loop replaced by PHYGRSGG | 0.012 | 0.018 | 0.67 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (VI), residues 44-51 of the 43-52 loop replaced by PHYGRSGG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (VII), residues 44-51 of the 43-52 loop replaced by PQRGRSAG | 0.006 | 0.0089 | 0.67 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (VII), residues 44-51 of the 43-52 loop replaced by PQRGRSAG |
| tissue-type plasminogen activator (t-PA) | — | staphylococcal nuclease variant (VIII), residues 44-51 of the 43-52 loop replaced by PGSGRSAG | 3.7 × 10⁻⁴ | 0.017 | 0.022 | 37 °C | Open record: tissue-type plasminogen activator (t-PA), staphylococcal nuclease variant (VIII), residues 44-51 of the 43-52 loop replaced by PGSGRSAG |
| trypsin | Bos taurus | staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA | 0.51 | 0.031 | 16 | 37 °C | Open record: trypsin, staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA |
| trypsin | Bos taurus | staphylococcal nuclease variant (II), residues 44-51 of the 43-52 loop replaced by PFGRSAG | 0.72 | 0.012 | 60 | 37 °C | Open record: trypsin, staphylococcal nuclease variant (II), residues 44-51 of the 43-52 loop replaced by PFGRSAG |
| trypsin | Bos taurus | staphylococcal nuclease variant (III), residues 44-51 of the 43-52 loop replaced by PPFGRSAG | 0.6 | 0.012 | 50 | 37 °C | Open record: trypsin, staphylococcal nuclease variant (III), residues 44-51 of the 43-52 loop replaced by PPFGRSAG |
| trypsin | Bos taurus | staphylococcal nuclease variant (IV), residues 44-51 of the 43-52 loop replaced by PGPFGRSAG | 1 | 0.011 | 90 | 37 °C | Open record: trypsin, staphylococcal nuclease variant (IV), residues 44-51 of the 43-52 loop replaced by PGPFGRSAG |
| trypsin | Bos taurus | staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG | 1.7 | 0.014 | 120 | 37 °C | Open record: trypsin, staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA | — | — | 0.0015 | 37 °C | Open record: urokinase-type plasminogen activator (u-PA), staphylococcal nuclease variant (I), residues 44-51 of the 43-52 loop replaced by PFGRSA |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | staphylococcal nuclease variant (IX), residues 44-51 of the 43-52 loop replaced by PGSGRSASGTTGTG | — | — | 0.9 | 37 °C | Open record: urokinase-type plasminogen activator (u-PA), staphylococcal nuclease variant (IX), residues 44-51 of the 43-52 loop replaced by PGSGRSASGTTGTG |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG | — | — | 0.025 | 37 °C | Open record: urokinase-type plasminogen activator (u-PA), staphylococcal nuclease variant (V), residues 44-51 of the 43-52 loop replaced by PGPFGRSAGG |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | staphylococcal nuclease variant (VI), residues 44-51 of the 43-52 loop replaced by PHYGRSGG | 8.8 × 10⁻⁵ | 0.03 | 0.0029 | 37 °C | Open record: urokinase-type plasminogen activator (u-PA), staphylococcal nuclease variant (VI), residues 44-51 of the 43-52 loop replaced by PHYGRSGG |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | staphylococcal nuclease variant (VII), residues 44-51 of the 43-52 loop replaced by PQRGRSAG | — | — | 0.0073 | 37 °C | Open record: urokinase-type plasminogen activator (u-PA), staphylococcal nuclease variant (VII), residues 44-51 of the 43-52 loop replaced by PQRGRSAG |
| urokinase-type plasminogen activator (u-PA) | Homo sapiens | staphylococcal nuclease variant (VIII), residues 44-51 of the 43-52 loop replaced by PGSGRSAG | 0.013 | 0.053 | 0.25 | 37 °C | Open record: urokinase-type plasminogen activator (u-PA), staphylococcal nuclease variant (VIII), residues 44-51 of the 43-52 loop replaced by PGSGRSAG |