CatLogenzyme kinetics catalog

Values CatLog read from this paper

3-Hydroxy-3-methylglutaryl-coenzyme A reductase of Haloferax volcanii: role of histidine 398 and attenuation of activity by introduction of negative charge at position 404

PMID 9007988doi:10.1002/pro.5560060118Open in Browse

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CatLog read 17 records (17 values) from this paper: 3-hydroxy-3-methylglutaryl-coenzyme A reductase from Haloferax volcanii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.34

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
3-hydroxy-3-methylglutaryl-coenzyme A reductaseHaloferax volcanii(S)-3-hydroxy-3-methylglutaryl-coenzyme A—0.06—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductaseHaloferax volcaniiNADPHreaction [1]; initiated with (S)-HMG-CoA—0.066—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductaseHaloferax volcaniiNADPHreaction [2]; reduction of (R,S)-mevaldehyde to mevalonate—0.032—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404DHaloferax volcanii(R,S)-3-hydroxy-3-methylglutaryl-coenzyme A—2—pH 7.3, 37 °COpen
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404DHaloferax volcaniiNADPH—0.16—pH 7.3, 37 °COpen
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404D/H398QHaloferax volcanii(R,S)-3-hydroxy-3-methylglutaryl-coenzyme A—0.083—pH 7.3, 37 °COpen
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404D/H398QHaloferax volcaniimevaldehyde—2.1—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404D/H398QHaloferax volcaniiNADPH—0.068—pH 7.3, 37 °COpen
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404D/H398QHaloferax volcaniiNADPH—0.084—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404NHaloferax volcanii(S)-3-hydroxy-3-methylglutaryl-coenzyme A—0.077—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404NHaloferax volcaniimevaldehyde—4.2—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404NHaloferax volcaniiNADPHreaction [1]; initiated with (S)-HMG-CoA—0.072—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant 404NHaloferax volcaniiNADPHreaction [2]; reduction of (R,S)-mevaldehyde to mevalonate—0.031—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant H398QHaloferax volcanii(R,S)-3-hydroxy-3-methylglutaryl-coenzyme A—0.2—pH 7.3, 37 °COpen
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant H398QHaloferax volcaniimevaldehyde—3—Open
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant H398QHaloferax volcaniiNADPH—0.177—pH 7.3, 37 °COpen
3-hydroxy-3-methylglutaryl-coenzyme A reductasevariant H398QHaloferax volcaniiNADPH—0.1—Open