Values CatLog read from this paper
Protein engineering tests of a homology model of Plasmodium falciparum lactate dehydrogenase
PMID 9051732doi:10.1093/protein/10.1.39Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (37 values) from this paper: lactate dehydrogenase (bsLDH) from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| lactate dehydrogenase (bsLDH) | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 0.33 | 6.6 | 0.05 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH), 4-methyl-2-oxopentanoate |
| lactate dehydrogenase (bsLDH) | Bacillus stearothermophilus | NADH | — | 0.018 | — | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH), NADH |
| lactate dehydrogenase (bsLDH) | Bacillus stearothermophilus | Phenylpyruvate | 39.5 | 0.775 | 51 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH), Phenylpyruvate |
| lactate dehydrogenase (bsLDH) | Bacillus stearothermophilus | Pyruvate | 180 | 0.06 | 3 × 10³ | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH), Pyruvate |
| lactate dehydrogenase (bsLDH) | Bacillus stearothermophilus | pyruvate | 177 | 0.056 | 3.2 × 10³ calc. | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH), pyruvate |
| lactate dehydrogenase (bsLDH)variant ELM1 | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 0.9 | 5.33 | 0.169 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant ELM1, 4-methyl-2-oxopentanoate |
| lactate dehydrogenase (bsLDH)variant ELM1 | Bacillus stearothermophilus | Phenylpyruvate | 108 | 1.28 | 84 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant ELM1, Phenylpyruvate |
| lactate dehydrogenase (bsLDH)variant ELM1 | Bacillus stearothermophilus | Pyruvate | 67.5 | 1.73 | 39 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant ELM1, Pyruvate |
| lactate dehydrogenase (bsLDH)variant ELM2 | Bacillus stearothermophilus | 4-methyl-2-oxopentanoate | 0.5 | 3.3 | 0.152 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant ELM2, 4-methyl-2-oxopentanoate |
| lactate dehydrogenase (bsLDH)variant ELM2 | Bacillus stearothermophilus | Phenylpyruvate | 39.5 | 0.506 | 78 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant ELM2, Phenylpyruvate |
| lactate dehydrogenase (bsLDH)variant ELM2 | Bacillus stearothermophilus | Pyruvate | 62.6 | 0.85 | 74 | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant ELM2, Pyruvate |
| lactate dehydrogenase (bsLDH)variant S163A | Bacillus stearothermophilus | pyruvate | 166 | 0.0998 | 1.66 × 10³ calc. | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant S163A, pyruvate |
| lactate dehydrogenase (bsLDH)variant S163L | Bacillus stearothermophilus | NADH | — | 0.065 | — | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant S163L, NADH |
| lactate dehydrogenase (bsLDH)variant S163L | Bacillus stearothermophilus | pyruvate | 101 | 6.33 | 16.0 calc. | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant S163L, pyruvate |
| lactate dehydrogenase (bsLDH)variant SLELM | Bacillus stearothermophilus | pyruvate | 1.5 | 12 | 0.12 calc. | pH 6, 25 °C | Open record: lactate dehydrogenase (bsLDH) variant SLELM, pyruvate |