Values CatLog read from this paper

Residues essential for catalysis and stability of the active site of Escherichia coli adenylosuccinate synthetase as revealed by directed mutation and kinetics

PMID 9115248doi:10.1074/jbc.272.18.11881Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (24 values) from this paper: adenylosuccinate synthetase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.4.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
adenylosuccinate synthetase	Escherichia coli	guanosine 5'-triphosphate	—	0.022	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, guanosine 5'-triphosphate
adenylosuccinate synthetase	Escherichia coli	guanosine 5'-triphosphate	—	0.013	—	pH 7, 25 °C	Open record: adenylosuccinate synthetase, guanosine 5'-triphosphate
adenylosuccinate synthetase	Escherichia coli	inosine 5'-monophosphate	—	0.021	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, inosine 5'-monophosphate
adenylosuccinate synthetase	Escherichia coli	inosine 5'-monophosphate	—	0.0131	—	pH 7, 25 °C	Open record: adenylosuccinate synthetase, inosine 5'-monophosphate
adenylosuccinate synthetase	Escherichia coli	L-aspartate	1.37	0.28	4.9 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, L-aspartate
adenylosuccinate synthetase	Escherichia coli	L-aspartate	1.92	0.228	8.42 calc.	pH 7, 25 °C	Open record: adenylosuccinate synthetase, L-aspartate
adenylosuccinate synthetasevariant E14A	Escherichia coli	guanosine 5'-triphosphate	—	0.031	—	pH 7, 25 °C	Open record: adenylosuccinate synthetase variant E14A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant E14A	Escherichia coli	inosine 5'-monophosphate	—	0.055	—	pH 7, 25 °C	Open record: adenylosuccinate synthetase variant E14A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant E14A	Escherichia coli	L-aspartate	0.022	1.4	0.016 calc.	pH 7, 25 °C	Open record: adenylosuccinate synthetase variant E14A, L-aspartate
adenylosuccinate synthetasevariant H41N	Escherichia coli	guanosine 5'-triphosphate	—	0.126	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant H41N, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant H41N	Escherichia coli	inosine 5'-monophosphate	—	0.0401	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant H41N, inosine 5'-monophosphate
adenylosuccinate synthetasevariant H41N	Escherichia coli	L-aspartate	0.00951	1.95	0.00488 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant H41N, L-aspartate
adenylosuccinate synthetasevariant K16Q	Escherichia coli	guanosine 5'-triphosphate	—	0.023	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant K16Q, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant K16Q	Escherichia coli	inosine 5'-monophosphate	—	0.0505	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant K16Q, inosine 5'-monophosphate
adenylosuccinate synthetasevariant K16Q	Escherichia coli	L-aspartate	0.479	0.308	1.56 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant K16Q, L-aspartate
adenylosuccinate synthetasevariant R131L	Escherichia coli	guanosine 5'-triphosphate	—	0.0352	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant R131L, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant R131L	Escherichia coli	inosine 5'-monophosphate	—	0.0355	—	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant R131L, inosine 5'-monophosphate
adenylosuccinate synthetasevariant R131L	Escherichia coli	L-aspartate	1.41	1	1.4 calc.	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant R131L, L-aspartate