Values CatLog read from this paper
Evolution of lactate dehydrogenase-A homologs of barracuda fishes (genus Sphyraena) from different thermal environments: differences in kinetic properties and thermal stability are due to amino acid…
PMID 9115998doi:10.1021/bi962664kOpen in Browse
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CatLog read 13 records (13 values) from this paper: lactate dehydrogenase-A (LDH-A) from Sphyraena lucasana, Sphyraena argentea, Sphyraena barracuda and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| lactate dehydrogenase-A (LDH-A) | Sphyraena argentea | pyruvate | — | 0.369 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A), pyruvate |
| lactate dehydrogenase-A (LDH-A) | Sphyraena argentea | β-nicotinamide adenine dinucleotide (reduced) | — | 0.0196 | — | 25 °C | Open record: lactate dehydrogenase-A (LDH-A), β-nicotinamide adenine dinucleotide (reduced) |
| lactate dehydrogenase-A (LDH-A) | Sphyraena barracuda | pyruvate | — | 0.19 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A), pyruvate |
| lactate dehydrogenase-A (LDH-A) | Sphyraena barracuda | β-nicotinamide adenine dinucleotide (reduced) | — | 0.0143 | — | 25 °C | Open record: lactate dehydrogenase-A (LDH-A), β-nicotinamide adenine dinucleotide (reduced) |
| lactate dehydrogenase-A (LDH-A) | Sphyraena ensis | β-nicotinamide adenine dinucleotide (reduced) | — | 0.0142 | — | 25 °C | Open record: lactate dehydrogenase-A (LDH-A), β-nicotinamide adenine dinucleotide (reduced) |
| lactate dehydrogenase-A (LDH-A) | Sphyraena helleri | pyruvate | — | 0.24 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A), pyruvate |
| lactate dehydrogenase-A (LDH-A) | Sphyraena helleri | β-nicotinamide adenine dinucleotide (reduced) | — | 0.016 | — | 25 °C | Open record: lactate dehydrogenase-A (LDH-A), β-nicotinamide adenine dinucleotide (reduced) |
| lactate dehydrogenase-A (LDH-A) | Sphyraena idiastes | β-nicotinamide adenine dinucleotide (reduced) | — | 0.0189 | — | 25 °C | Open record: lactate dehydrogenase-A (LDH-A), β-nicotinamide adenine dinucleotide (reduced) |
| lactate dehydrogenase-A (LDH-A) | Sphyraena lucasana | pyruvatenative | — | 0.258 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A), pyruvate |
| lactate dehydrogenase-A (LDH-A) | Sphyraena lucasana | pyruvaterecombinant; expressed in E. coli BL21 (pGEMEX vector) | — | 0.26 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A), pyruvate |
| lactate dehydrogenase-A (LDH-A) | Sphyraena lucasana | β-nicotinamide adenine dinucleotide (reduced) | — | 0.0186 | — | 25 °C | Open record: lactate dehydrogenase-A (LDH-A), β-nicotinamide adenine dinucleotide (reduced) |
| lactate dehydrogenase-A (LDH-A)variant D8G (C-8) | Sphyraena lucasana | pyruvate | — | 0.283 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A) variant D8G (C-8), pyruvate |
| lactate dehydrogenase-A (LDH-A)variant V61A/G68S (C-61-68) | Sphyraena lucasana | pyruvate | — | 0.37 | — | pH 6.98, 25 °C | Open record: lactate dehydrogenase-A (LDH-A) variant V61A/G68S (C-61-68), pyruvate |