Values CatLog read from this paper
Design of an active fragment of a class II aminoacyl-tRNA synthetase and its significance for synthetase evolution
PMID 9131996doi:10.1021/bi962395yOpen in Browse
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CatLog read 16 records (45 values) from this paper: histidyl-tRNA synthetase (HisRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphatefull-length; untagged | 120 | 0.89 | 130 | pH 8, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphatefull-length; His6-tagged (affinity-tagged full-length HisRS | 110 | 0.6 | 180 | pH 8, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphateN-terminal MRGS(His)6 tag (pQE-30) | 0.17 | 0.74 | 0.23 | pH 8, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA)full-length; His6-tagged (affinity-tagged full-length HisRS | 1.5 | 0.013 | 110 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA) |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA)N-terminal MRGS(His)6 tag (pQE-30) | 0.0026 | 0.0055 | 0.48 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA) |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcriptfull-length; His6-tagged (affinity-tagged full-length HisRS | 0.026 | 0.0038 | 6.8 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcriptN-terminal MRGS(His)6 tag (pQE-30) | 1.1 × 10⁻⁴ | 0.0044 | 0.026 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidinefull-length; untagged | 142 | 0.03 | 4.7 × 10³ | pH 8, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidinefull-length; His6-tagged (affinity-tagged full-length HisRS | 140 | 0.014 | 9.9 × 10³ | pH 8, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidineN-terminal MRGS(His)6 tag (pQE-30) | 0.19 | 0.046 | 4.1 | pH 8, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | tRNAHis transcript with U73 discriminator base (C73U), anticodon GUGfull-length; His6-tagged (affinity-tagged full-length HisRS | 0.005 | 0.0016 | 3.1 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), tRNAHis transcript with U73 discriminator base (C73U), anticodon GUG |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | tRNAHis transcript with U73 discriminator base (C73U), anticodon GUGN-terminal MRGS(His)6 tag (pQE-30) | 1.6 × 10⁻⁴ | 3.1 × 10⁻⁴ | 0.53 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), tRNAHis transcript with U73 discriminator base (C73U), anticodon GUG |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptwt tRNAHis varied 0.1-10 µM; full-length | 2.6 | 0.0014 | 1.8 × 10³ | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptwt tRNAHis varied 0.1-10 µM | 0.0064 | 5.6 × 10⁻⁴ | 11 | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptwt tRNAHis varied; N-terminal MRGS(His)6 tag (pQE-30) | 0.004 | 1.9 × 10⁻⁴ | 21 calc. | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptkcat determined at 10-500 nM Ncat HisRS (Figure 6A) | 0.006 | — | — | pH 7.5, 37 °C | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |