EC 6.1.1.21: histidine-tRNA ligase
LigasesOpen in Browse
CatLog read 39 records for EC 6.1.1.21 from 3 papers, for 2 organisms. Names in these papers: histidyl-tRNA synthetase (HisRS), histidyl-tRNA synthetase (HARS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 34 records for EC 6.1.1.21 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.21 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Design of an active fragment of a class II aminoacyl-tRNA synthetase and its significance for synthetase evolution | Escherichia coli | 16 |
| Isolation and analysis of mutated histidyl-tRNA synthetases from Escherichia coli | Escherichia coli | 13 |
| Substrate interaction defects in histidyl-tRNA synthetase linked to dominant axonal peripheral neuropathy | Homo sapiens | 10 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| histidyl-tRNA synthetase (HARS) | Homo sapiens | ATP | 5.8 | 0.0442 | 130 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS), ATP |
| histidyl-tRNA synthetase (HARS)variant S356N | Homo sapiens | ATP | — | — | 0.15 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant S356N, ATP |
| histidyl-tRNA synthetase (HARS)variant S356N | Homo sapiens | histidine | 0.39 | 0.0108 | 40 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant S356N, histidine |
| histidyl-tRNA synthetase (HARS)variant S356N | Homo sapiens | tRNAHis | 0.3 | 1.99 × 10⁻⁴ | 1.5 × 10³ | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant S356N, tRNAHis |
| histidyl-tRNA synthetase (HARS)variant V155G | Homo sapiens | ATP | — | — | 0.38 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant V155G, ATP |
| histidyl-tRNA synthetase (HARS)variant V155G | Homo sapiens | histidine | 2.08 | 0.6872 | 3 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant V155G, histidine |
| histidyl-tRNA synthetase (HARS)variant V155G | Homo sapiens | tRNAHis | 3.05 | 9.79 × 10⁻⁴ | 3.1 × 10³ | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant V155G, tRNAHis |
| histidyl-tRNA synthetase (HARS)variant Y330C | Homo sapiens | ATP | 0.48 | 1.763 | 0.272 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant Y330C, ATP |
| histidyl-tRNA synthetase (HARS)variant Y330C | Homo sapiens | histidine | 0.74 | 0.2029 | 4 | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant Y330C, histidine |
| histidyl-tRNA synthetase (HARS)variant Y330C | Homo sapiens | tRNAHis | 0.56 | 3.3 × 10⁻⁴ | 1.7 × 10³ | pH 7.5 | PMID 29235198 | Open record: histidyl-tRNA synthetase (HARS) variant Y330C, tRNAHis |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphatefull-length; untagged | 120 | 0.89 | 130 | pH 8, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphatefull-length; His6-tagged (affinity-tagged full-length HisRS | 110 | 0.6 | 180 | pH 8, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphateN-terminal MRGS(His)6 tag (pQE-30) | 0.17 | 0.74 | 0.23 | pH 8, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | adenosine 5'-triphosphate | 34 | 0.56 | 61 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA)full-length; His6-tagged (affinity-tagged full-length HisRS | 1.5 | 0.013 | 110 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA) |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA)N-terminal MRGS(His)6 tag (pQE-30) | 0.0026 | 0.0055 | 0.48 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), amber suppressor tRNAHis transcript (GUG anticodon replaced by CUA) |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcriptfull-length; His6-tagged (affinity-tagged full-length HisRS | 0.026 | 0.0038 | 6.8 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcriptN-terminal MRGS(His)6 tag (pQE-30) | 1.1 × 10⁻⁴ | 0.0044 | 0.026 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), eight-base-pair microhelixHis (acceptor helix of tRNAHis) transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidinefull-length; untagged | 142 | 0.03 | 4.7 × 10³ | pH 8, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidinefull-length; His6-tagged (affinity-tagged full-length HisRS | 140 | 0.014 | 9.9 × 10³ | pH 8, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidineN-terminal MRGS(His)6 tag (pQE-30) | 0.19 | 0.046 | 4.1 | pH 8, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | L-histidine | 7 | 0.008 | 815 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS), L-histidine |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | tRNAHis transcript with U73 discriminator base (C73U), anticodon GUGfull-length; His6-tagged (affinity-tagged full-length HisRS | 0.005 | 0.0016 | 3.1 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), tRNAHis transcript with U73 discriminator base (C73U), anticodon GUG |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | tRNAHis transcript with U73 discriminator base (C73U), anticodon GUGN-terminal MRGS(His)6 tag (pQE-30) | 1.6 × 10⁻⁴ | 3.1 × 10⁻⁴ | 0.53 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), tRNAHis transcript with U73 discriminator base (C73U), anticodon GUG |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptwt tRNAHis varied 0.1-10 µM; full-length | 2.6 | 0.0014 | 1.8 × 10³ | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptwt tRNAHis varied 0.1-10 µM | 0.0064 | 5.6 × 10⁻⁴ | 11 | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptwt tRNAHis varied; N-terminal MRGS(His)6 tag (pQE-30) | 0.004 | 1.9 × 10⁻⁴ | 21 calc. | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS) | Escherichia coli | wild-type tRNAHis (anticodon GUG) in vitro T7 transcriptkcat determined at 10-500 nM Ncat HisRS (Figure 6A) | 0.006 | — | — | pH 7.5, 37 °C | PMID 9131996 | Open record: histidyl-tRNA synthetase (HisRS), wild-type tRNAHis (anticodon GUG) in vitro T7 transcript |
| histidyl-tRNA synthetase (HisRS)variant M-HisRS | Escherichia coli | adenosine 5'-triphosphate | 9 | 0.39 | 24 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant M-HisRS, adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS)variant M-HisRS | Escherichia coli | L-histidine | 6 | 0.011 | 550 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant M-HisRS, L-histidine |
| histidyl-tRNA synthetase (HisRS)variant R259K | Escherichia coli | adenosine 5'-triphosphate | 0.5 | 0.3 | 1.7 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant R259K, adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS)variant R259K | Escherichia coli | L-histidine | 0.1 | 0.017 | 6 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant R259K, L-histidine |
| histidyl-tRNA synthetase (HisRS)variant R259Q | Escherichia coli | adenosine 5'-triphosphate | 0.4 | 0.33 | 1.2 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant R259Q, adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS)variant V-HisRS | Escherichia coli | adenosine 5'-triphosphate | 8 | 0.35 | 22 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant V-HisRS, adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS)variant V-HisRS | Escherichia coli | L-histidine | 6 | 0.017 | 370 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant V-HisRS, L-histidine |
| histidyl-tRNA synthetase (HisRS)variant des(A2-G10) | Escherichia coli | adenosine 5'-triphosphate | 0.2 | 0.655 | 3 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant des(A2-G10), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS)variant des(A2-G10) | Escherichia coli | L-histidine | 1 | 0.06 | 15 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant des(A2-G10), L-histidine |
| histidyl-tRNA synthetase (HisRS)variant des(A2-Q6) | Escherichia coli | adenosine 5'-triphosphate | 8 | 0.22 | 30 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant des(A2-Q6), adenosine 5'-triphosphate |
| histidyl-tRNA synthetase (HisRS)variant des(A2-Q6) | Escherichia coli | L-histidine | 9 | 0.02 | 450 | pH 7.4, 37 °C | PMID 9266856 | Open record: histidyl-tRNA synthetase (HisRS) variant des(A2-Q6), L-histidine |