CatLogenzyme kinetics catalog

Values CatLog read from this paper

Site-directed mutagenesis of human leukotriene C4 synthase

PMID 9153254doi:10.1074/jbc.272.21.13923Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 36 records (36 values) from this paper: leukotriene C4 synthase (LTC_4S) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.4.1.20

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
leukotriene C4 synthase (LTC_4S)Homo sapiensglutathione (reduced)—1.4—pH 7.6Open
leukotriene C4 synthase (LTC_4S)Homo sapiensleukotriene A4 methyl ester—0.0074—pH 7.6Open
leukotriene C4 synthase (LTC_4S)hybrid A (LTC4S/FLAP chimera: third hydrophobic domain and carboxyl terminus of FLAP)Homo sapiensleukotriene A4 methyl ester—0.0056—pH 7.6Open
leukotriene C4 synthase (LTC_4S)hybrid C (LTC4S/FLAP chimera: residues 66-81 replaced by FLAP residues 70-85, plus P78L)Homo sapiensleukotriene A4 methyl ester—0.0095—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant A52SHomo sapiensglutathione (reduced)—30—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant A52SHomo sapiensleukotriene A4 methyl ester—0.0045—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant C82VHomo sapiensglutathione (reduced)assay on solubilized COS cell microsomes; 0.4% Triton X-102—2—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant C82VHomo sapiensglutathione (reduced)3-100 ng purified enzyme per assay—0.9—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant C82VHomo sapiensleukotriene A4 methyl esterassay on solubilized COS cell microsomes; 0.4% Triton X-102—0.0109—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant C82VHomo sapiensleukotriene A4 methyl ester3-100 ng purified enzyme per assay—0.0063—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant L62THomo sapiensglutathione (reduced)—2.5—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant L62THomo sapiensleukotriene A4 methyl ester—0.0067—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant N55AHomo sapiensglutathione (reduced)—10.7—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant N55AHomo sapiensleukotriene A4 methyl ester—0.0077—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51HHomo sapiensglutathione (reduced)assay on solubilized COS cell microsomes; 0.4% Triton X-102—3.8—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51HHomo sapiensglutathione (reduced)3-100 ng purified enzyme per assay—1.5—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51HHomo sapiensleukotriene A4 methyl esterassay on solubilized COS cell microsomes; 0.4% Triton X-102—0.0064—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51HHomo sapiensleukotriene A4 methyl ester3-100 ng purified enzyme per assay—0.0063—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51I/wild-type covalent pseudo-heterodimerHomo sapiensglutathione (reduced)—3.2—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51I/wild-type covalent pseudo-heterodimerHomo sapiensleukotriene A4 methyl ester—0.0065—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51KHomo sapiensglutathione (reduced)—2.3—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant R51KHomo sapiensleukotriene A4 methyl ester—0.0094—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant V49FHomo sapiensglutathione (reduced)—9.2—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant V49FHomo sapiensleukotriene A4 methyl ester—0.01—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant V69THomo sapiensglutathione (reduced)—2—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant V69THomo sapiensleukotriene A4 methyl ester—0.019—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y109IHomo sapiensglutathione (reduced)—1.6—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y109IHomo sapiensleukotriene A4 methyl ester—0.02—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y59FHomo sapiensglutathione (reduced)—15.5—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y59FHomo sapiensleukotriene A4 methyl ester—0.019—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y93FHomo sapiensglutathione (reduced)assay on solubilized COS cell microsomes; 0.4% Triton X-102—10—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y93FHomo sapiensglutathione (reduced)3-100 ng purified enzyme per assay—9.9—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y93FHomo sapiensleukotriene A4 methyl esterassay on solubilized COS cell microsomes; 0.4% Triton X-102—0.02—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y93FHomo sapiensleukotriene A4 methyl ester3-100 ng purified enzyme per assay—0.0054—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y97FHomo sapiensglutathione (reduced)—16.5—pH 7.6Open
leukotriene C4 synthase (LTC_4S)variant Y97FHomo sapiensleukotriene A4 methyl ester—0.0165—pH 7.6Open