Values CatLog read from this paper
The roles of conserved carboxylate residues in IMP dehydrogenase and identification of a transition state analog
PMID 9341229doi:10.1021/bi9714161Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (36 values) from this paper: IMP dehydrogenase (IMPDH) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| IMP dehydrogenase (IMPDH) | Escherichia coli | inosine 5'-monophosphate | 13 | 0.061 | 210 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH), inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH) | Escherichia coli | nicotinamide adenine dinucleotide | — | 2 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH), nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D138A | Escherichia coli | inosine 5'-monophosphate | 7.1 | 0.044 | 160 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D138A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D138A | Escherichia coli | nicotinamide adenine dinucleotide | — | 1.4 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D138A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D13A | Escherichia coli | inosine 5'-monophosphate | 0.7 | 0.052 | 13 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D13A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D13A | Escherichia coli | nicotinamide adenine dinucleotide | — | 5.7 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D13A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D200A | Escherichia coli | inosine 5'-monophosphate | 14 | 0.044 | 320 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D200A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D200A | Escherichia coli | nicotinamide adenine dinucleotide | — | 1.5 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D200A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D243A | Escherichia coli | inosine 5'-monophosphate | 12 | 0.056 | 210 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D243A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D243A | Escherichia coli | nicotinamide adenine dinucleotide | — | 2 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D243A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D248A | Escherichia coli | inosine 5'-monophosphate | 0.1 | 0.021 | 4.8 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D248A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D248A | Escherichia coli | nicotinamide adenine dinucleotide | — | 6.3 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D248A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D338A | Escherichia coli | inosine 5'-monophosphate | 0.02 | 0.71 | 0.028 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D338A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D338A | Escherichia coli | nicotinamide adenine dinucleotide | — | 3.3 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D338A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant D50A | Escherichia coli | inosine 5'-monophosphate | 1.2 | 0.061 | 20 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D50A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant D50A | Escherichia coli | nicotinamide adenine dinucleotide | — | 4.1 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant D50A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant E369A | Escherichia coli | inosine 5'-monophosphate | 7.1 | 0.053 | 130 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E369A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant E369A | Escherichia coli | nicotinamide adenine dinucleotide | — | 2 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E369A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant E373A | Escherichia coli | inosine 5'-monophosphate | 7.1 | 0.059 | 120 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E373A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant E373A | Escherichia coli | nicotinamide adenine dinucleotide | — | 1.6 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E373A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant E469A | Escherichia coli | inosine 5'-monophosphate | 14 | 0.049 | 290 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E469A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant E469A | Escherichia coli | nicotinamide adenine dinucleotide | — | 11 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E469A, nicotinamide adenine dinucleotide |
| IMP dehydrogenase (IMPDH)variant E54A | Escherichia coli | inosine 5'-monophosphate | 14 | 0.087 | 160 calc. | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E54A, inosine 5'-monophosphate |
| IMP dehydrogenase (IMPDH)variant E54A | Escherichia coli | nicotinamide adenine dinucleotide | — | 2.4 | — | pH 8, 25 °C | Open record: IMP dehydrogenase (IMPDH) variant E54A, nicotinamide adenine dinucleotide |