Values CatLog read from this paper
Expression and characterization of recombinant type 2 3 alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3 alpha/17 beta-HSD activity and cellular…
PMID 9415401doi:10.1210/mend.11.13.0026Open in Browse
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CatLog read 11 records (33 values) from this paper: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 17beta-hydroxy-5alpha-androstan-3-one | 0.0018 | 0.019 | 0.0965 | pH 7, 25 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 17beta-hydroxy-5alpha-androstan-3-one |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 17beta-hydroxy-5alpha-androstan-3-oneradiometric assay ([14C]-steroid, TLC); NADPH 2.3 mM | 0.003 | 0.0262 | 0.1145 | pH 7, 37 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 17beta-hydroxy-5alpha-androstan-3-one |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 17beta-hydroxy-5alpha-androstan-3-oneradiometric assay (TLC); NADP+ 2.3 mM; cosubstrate NADP+ | 9.7 × 10⁻⁴ | 0.0243 | 0.0398 | pH 7, 37 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 17beta-hydroxy-5alpha-androstan-3-one |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 17beta-hydroxyandrost-4-en-3-one | 9 × 10⁻⁴ | 0.0119 | 0.0757 | pH 7, 37 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 17beta-hydroxyandrost-4-en-3-one |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 3alpha-hydroxy-5alpha-androstan-17-one | 3.7 × 10⁻⁴ | 0.0484 | 0.0078 | pH 7, 37 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 3alpha-hydroxy-5alpha-androstan-17-one |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 4-Nitrobenzaldehyde | 0.0942 | 0.0873 | 1.078 | pH 7, 25 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 4-Nitrobenzaldehyde |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 5alpha-androstane-3,17-dione | 0.008 | 0.00422 | 1.9 | pH 7, 25 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 5alpha-androstane-3,17-dione |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 5alpha-androstane-3alpha,17beta-diolspectrophotometric assay (A340); NADP+ fixed at 2.3 mM | 0.0035 | 0.0171 | 0.203 | pH 7, 25 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 5alpha-androstane-3alpha,17beta-diol |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 5alpha-androstane-3alpha,17beta-diolNADP+ 2.3 mM | 0.0097 | 0.0191 | 0.507 | pH 7, 25 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 5alpha-androstane-3alpha,17beta-diol |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 5alpha-androstane-3alpha,17beta-diol | 0.0062 | 0.0193 | 0.32 | pH 7, 37 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 5alpha-androstane-3alpha,17beta-diol |
| type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3) | Homo sapiens | 9,10-Phenanthrenequinone | 0.39 | 0.0206 | 18.93 | pH 7, 25 °C | Open record: type 2 3α-hydroxysteroid dehydrogenase (3α/17β-HSD, AKR1C3), 9,10-Phenanthrenequinone |