Values CatLog read from this paper
Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400-->Cys of glucoamylase from Aspergillus awamori
PMID 9521694doi:10.1021/bi972232pOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 33 records (99 values) from this paper: glucoamylase from Aspergillus awamori.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glucoamylase | Aspergillus awamori | 4-nitrophenyl alpha-D-glucopyranoside | 0.25 | 3.4 | 0.073 | pH 4.5, 45 °C | Open record: glucoamylase, 4-nitrophenyl alpha-D-glucopyranoside |
| glucoamylase | Aspergillus awamori | alpha-D-glucopyranosyl fluoride | 300 | 70 | 4.2 | pH 4.5, 45 °C | Open record: glucoamylase, alpha-D-glucopyranosyl fluoride |
| glucoamylase | Aspergillus awamori | isomaltoheptaose | 1.3 | 17 | 0.077 | pH 4.5, 45 °C | Open record: glucoamylase, isomaltoheptaose |
| glucoamylase | Aspergillus awamori | isomaltohexaose | 1.5 | 18 | 0.081 | pH 4.5, 45 °C | Open record: glucoamylase, isomaltohexaose |
| glucoamylase | Aspergillus awamori | isomaltopentaose | 1.2 | 16 | 0.077 | pH 4.5, 45 °C | Open record: glucoamylase, isomaltopentaose |
| glucoamylase | Aspergillus awamori | isomaltotetraose | 0.99 | 15 | 0.069 | pH 4.5, 45 °C | Open record: glucoamylase, isomaltotetraose |
| glucoamylase | Aspergillus awamori | isomaltotriose | 0.64 | 18 | 0.036 | pH 4.5, 45 °C | Open record: glucoamylase, isomaltotriose |
| glucoamylase | Aspergillus awamori | kojibiose | 0.13 | 150 | 8.4 × 10⁻⁴ | pH 4.5, 45 °C | Open record: glucoamylase, kojibiose |
| glucoamylase | Aspergillus awamori | nigerose | 0.14 | 32 | 0.0044 | pH 4.5, 45 °C | Open record: glucoamylase, nigerose |
| glucoamylase | Aspergillus awamori | panose | 6.9 | 17 | 0.41 | pH 4.5, 45 °C | Open record: glucoamylase, panose |
| glucoamylasevariant C320A/E400C (Cys400 GA) | Aspergillus awamori | maltoheptaose | 0.12 | 0.45 | 0.26 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C (Cys400 GA), maltoheptaose |
| glucoamylasevariant C320A/E400C (Cys400 GA) | Aspergillus awamori | maltohexaose | 0.12 | 0.51 | 0.23 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C (Cys400 GA), maltohexaose |
| glucoamylasevariant C320A/E400C (Cys400 GA) | Aspergillus awamori | maltopentaose | 0.12 | 0.49 | 0.24 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C (Cys400 GA), maltopentaose |
| glucoamylasevariant C320A/E400C (Cys400 GA) | Aspergillus awamori | maltose | 0.015 | 2.7 | 0.0055 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C (Cys400 GA), maltose |
| glucoamylasevariant C320A/E400C (Cys400 GA) | Aspergillus awamori | maltotetraose | 0.11 | 0.61 | 0.19 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C (Cys400 GA), maltotetraose |
| glucoamylasevariant C320A/E400C (Cys400 GA) | Aspergillus awamori | maltotriose | 0.062 | 1.1 | 0.058 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C (Cys400 GA), maltotriose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | 4-nitrophenyl alpha-D-glucopyranoside | 0.73 | 4 | 0.18 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), 4-nitrophenyl alpha-D-glucopyranoside |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | alpha-D-glucopyranosyl fluoride | 970 | 110 | 8.9 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), alpha-D-glucopyranosyl fluoride |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | isomaltoheptaose | 3.9 | 37 | 0.107 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), isomaltoheptaose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | isomaltohexaose | 4.3 | 42 | 0.102 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), isomaltohexaose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | isomaltopentaose | 3.9 | 38 | 0.102 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), isomaltopentaose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | isomaltose | 0.23 | 32 | 0.0072 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), isomaltose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | isomaltotetraose | 2.6 | 36 | 0.073 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), isomaltotetraose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | isomaltotriose | 1.4 | 32 | 0.043 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), isomaltotriose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | kojibiose | 0.14 | 120 | 0.0012 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), kojibiose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | maltoheptaose | 86 | 0.45 | 190 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), maltoheptaose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | maltohexaose | 89 | 0.55 | 160 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), maltohexaose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | maltopentaose | 83 | 0.58 | 140 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), maltopentaose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | maltose | 10.4 | 2.5 | 4.2 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), maltose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | maltotetraose | 76 | 0.68 | 110 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), maltotetraose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | maltotriose | 42 | 0.96 | 44 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), maltotriose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | nigerose | 0.28 | 92 | 0.0031 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), nigerose |
| glucoamylasevariant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA) | Aspergillus awamori | panose | 14 | 42 | 0.33 | pH 4.5, 45 °C | Open record: glucoamylase variant C320A/E400C, Cys400 oxidized to cysteinesulfinic acid (Cys400-SO2H GA), panose |