Values CatLog read from this paper

Ambiguities in mapping the active site of a conformationally dynamic enzyme by directed mutation. Role of dynamics in structure-function correlations in Escherichia coli adenylosuccinate synthetase

PMID 9632649doi:10.1074/jbc.273.26.16000Open in Browse

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CatLog read 28 records (64 values) from this paper: adenylosuccinate synthetase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.3.4.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
adenylosuccinate synthetase	Escherichia coli	guanosine 5'-triphosphate	—	0.026	38	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, guanosine 5'-triphosphate
adenylosuccinate synthetase	Escherichia coli	guanosine 5'-triphosphate	—	0.011	9	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase, guanosine 5'-triphosphate
adenylosuccinate synthetase	Escherichia coli	inosine 5'-monophosphate	1	0.028	36	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, inosine 5'-monophosphate
adenylosuccinate synthetase	Escherichia coli	inosine 5'-monophosphate	0.096	0.07	1.4	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase, inosine 5'-monophosphate
adenylosuccinate synthetase	Escherichia coli	L-aspartate	—	0.23	4.4	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase, L-aspartate
adenylosuccinate synthetase	Escherichia coli	L-aspartate	—	0.9	0.11	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase, L-aspartate
adenylosuccinate synthetasevariant D21A	Escherichia coli	guanosine 5'-triphosphate	—	0.052	0.95	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant D21A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant D21A	Escherichia coli	inosine 5'-monophosphate	0.049	0.048	1.02	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant D21A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant D21A	Escherichia coli	L-aspartate	—	0.17	0.3	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant D21A, L-aspartate
adenylosuccinate synthetasevariant N38A	Escherichia coli	guanosine 5'-triphosphate	—	0.041	0.12	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant N38A	Escherichia coli	inosine 5'-monophosphate	0.0048	0.049	0.1	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant N38A	Escherichia coli	L-aspartate	—	0.3	0.016	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38A, L-aspartate
adenylosuccinate synthetasevariant N38D	Escherichia coli	guanosine 5'-triphosphate	—	0.27	0.051	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38D, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant N38D	Escherichia coli	guanosine 5'-triphosphate	—	0.116	1.5	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase variant N38D, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant N38D	Escherichia coli	inosine 5'-monophosphate	0.0134	1.6	0.008	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38D, inosine 5'-monophosphate
adenylosuccinate synthetasevariant N38D	Escherichia coli	inosine 5'-monophosphatespectrophotometric assay; absorbance at 290 nm	0.171	3.8	0.045	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase variant N38D, inosine 5'-monophosphate
adenylosuccinate synthetasevariant N38D	Escherichia coli	inosine 5'-monophosphatekcat-vs-pH profile (Figure 3); 20 mM MES/HEPES buffer	0.237	—	—	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase variant N38D, inosine 5'-monophosphate
adenylosuccinate synthetasevariant N38D	Escherichia coli	L-aspartate	—	2.6	0.0052	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38D, L-aspartate
adenylosuccinate synthetasevariant N38D	Escherichia coli	L-aspartate	—	0.5	0.33	pH 5.6, 25 °C	Open record: adenylosuccinate synthetase variant N38D, L-aspartate
adenylosuccinate synthetasevariant N38E	Escherichia coli	guanosine 5'-triphosphate	—	0.054	0.62	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38E, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant N38E	Escherichia coli	inosine 5'-monophosphate	0.034	0.028	1.22	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38E, inosine 5'-monophosphate
adenylosuccinate synthetasevariant N38E	Escherichia coli	L-aspartate	—	0.24	0.143	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant N38E, L-aspartate
adenylosuccinate synthetasevariant R419L	Escherichia coli	guanosine 5'-triphosphate	—	0.25	0.41	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant R419L, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant R419L	Escherichia coli	inosine 5'-monophosphate	0.1	0.2	0.49	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant R419L, inosine 5'-monophosphate
adenylosuccinate synthetasevariant R419L	Escherichia coli	L-aspartate	—	0.34	0.29	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant R419L, L-aspartate
adenylosuccinate synthetasevariant T42A	Escherichia coli	guanosine 5'-triphosphate	—	0.28	3.2	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T42A, guanosine 5'-triphosphate
adenylosuccinate synthetasevariant T42A	Escherichia coli	inosine 5'-monophosphate	0.89	0.123	7.2	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T42A, inosine 5'-monophosphate
adenylosuccinate synthetasevariant T42A	Escherichia coli	L-aspartate	—	1.4	0.65	pH 7.7, 25 °C	Open record: adenylosuccinate synthetase variant T42A, L-aspartate