Values CatLog read from this paper
His68 and His141 are critical contributors to the intersubunit catalytic site of adenylosuccinate lyase of Bacillus subtilis
PMID 9890879doi:10.1021/bi982299sOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (15 values) from this paper: adenylosuccinate lyase from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| adenylosuccinate lyase | Bacillus subtilis | adenylosuccinate | 1.7 | 0.0026 | 650 | pH 7, 25 °C | Open record: adenylosuccinate lyase, adenylosuccinate |
| adenylosuccinate lyasevariant H141A + H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0063 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141A + H68Q (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141E + H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0079 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141E + H68Q (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141L + H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0055 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141L + H68Q (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141Q + H68A (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0175 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141Q + H68A (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141Q + H68K (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0101 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141Q + H68K (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141Q + H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0084 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141Q + H68Q (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141Q + H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0092 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141Q + H68Q (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141Q + R67M:H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0123 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141Q + R67M:H68Q (hybrid), adenylosuccinate |
| adenylosuccinate lyasevariant H141R | Bacillus subtilis | adenylosuccinate | 2.8 × 10⁻⁵ | 0.0067 | 0.0042 | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141R, adenylosuccinate |
| adenylosuccinate lyasevariant H141R + H68Q (hybrid) | Bacillus subtilis | adenylosuccinate | — | 0.0133 | — | pH 7, 25 °C | Open record: adenylosuccinate lyase variant H141R + H68Q (hybrid), adenylosuccinate |