Values CatLog read from this paper
Engineering, characterization and phage display of hepatitis C virus NS3 protease and NS4A cofactor peptide as a single-chain protein
PMID 9930676doi:10.1093/protein/11.12.1257Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (16 values) from this paper: NS3 protease from hepatitis C virus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | — | 0.088 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | 0.0253 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | — | 0.519 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | 0.03 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | — | 0.087 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | 0.0255 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | — | 0.502 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | 0.027 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | — | 0.102 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | 0.0017 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | — | 0.49 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | 0.0018 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | — | 0.063 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | 0.023 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | — | 0.403 | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | 0.027 | — | — | pH 7.5, 25 °C | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |