EC 3.4.21.98: hepacivirin
HydrolasesOpen in Browse
CatLog read 37 records for EC 3.4.21.98 from 3 papers, for 2 organisms. Names in these papers: NS3 protease, NS3/NS4A serine protease (single-chain NS3 protease domain), NS3–NS4A protease (genotype 1b, Con1-R3), NS3–NS4A protease (genotype 2a, JFH1) and 3 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 47 records for EC 3.4.21.98 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.98 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Impact of naturally occurring variants of HCV protease on the binding of different classes of protease inhibitors | Hepatitis C virus | 16 |
| Engineering, characterization and phage display of hepatitis C virus NS3 protease and NS4A cofactor peptide as a single-chain protein | hepatitis C virus | 16 |
| Protease and helicase activities of hepatitis C virus genotype 4, 5, and 6 NS3-NS4A proteins | Hepatitis C virus | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | — | 0.088 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | 0.0253 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | — | 0.519 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | 0.03 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | — | 0.087 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; C-terminal GS(H)6 tag | 0.0255 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | — | 0.502 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; C-terminal GS(H)6 tag | 0.027 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | — | 0.102 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | 0.0017 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | — | 0.49 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | 0.0018 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | — | 0.063 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)base buffer; no added NaCl; NS3 protease domain | 0.023 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | — | 0.403 | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3 protease | hepatitis C virus | acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site)buffer containing 150 mM NaCl; NS3 protease domain | 0.027 | — | — | pH 7.5, 25 °C | PMID 9930676 | Open record: NS3 protease, acetyl-Asp-Glu-Glu-Met-Glu-Cys-Ala-Ser-His-Leu-Pro-Tyr-Lys-amide (13-mer peptide of the HCV NS4A/NS4B cleavage site) |
| NS3/NS4A serine protease (single-chain NS3 protease domain) | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.4 | 0.0039 | 100 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain), Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain) | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.43 | 0.0026 | 170 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain), Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain) | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.57 | 0.0072 | 79 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain), Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant D168Q | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.33 | 0.0042 | 79 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant D168Q, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant D79E | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.22 | 0.0058 | 37 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant D79E, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant Q80G | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.22 | 0.0055 | 39 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant Q80G, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant R123T | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.13 | 0.012 | 11 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant R123T, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant R123T/D168Q | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.25 | 0.0051 | 49 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant R123T/D168Q, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant R123T/D168Q/V132L | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.3 | 0.005 | 60 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant R123T/D168Q/V132L, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant S122K | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.58 | 0.0048 | 120 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant S122K, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant S122K/V132L | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.25 | 0.0044 | 57 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant S122K/V132L, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant T160A | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.2 | 0.005 | 40 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant T160A, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant V132L | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.095 | 0.0034 | 28 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant V132L, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant V78A | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.3 | 0.0059 | 51 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant V78A, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant V78A/D79E/Q80G | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.17 | 0.0073 | 23 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant V78A/D79E/Q80G, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3/NS4A serine protease (single-chain NS3 protease domain)variant V78A/D79E/Q80G/S122K/V132L | Hepatitis C virus | Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester | 0.13 | 0.0047 | 28 calc. | pH 6.5, 30 °C | PMID 16445277 | Open record: NS3/NS4A serine protease (single-chain NS3 protease domain) variant V78A/D79E/Q80G/S122K/V132L, Ac-DTEDVVP(Nva)-O-4-phenylazophenyl ester |
| NS3–NS4A protease (genotype 1b, Con1-R3) | Hepatitis C virus | anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH | 0.77 | 0.0021 | 370 | pH 8, 23 °C | PMID 19944069 | Open record: NS3–NS4A protease (genotype 1b, Con1-R3), anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH |
| NS3–NS4A protease (genotype 2a, JFH1) | Hepatitis C virus | anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH | 0.8 | 0.0035 | 230 | pH 8, 23 °C | PMID 19944069 | Open record: NS3–NS4A protease (genotype 2a, JFH1), anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH |
| NS3–NS4A protease (genotype 4a) | Hepatitis C virus | anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH | 0.21 | 0.0013 | 160 | pH 8, 23 °C | PMID 19944069 | Open record: NS3–NS4A protease (genotype 4a), anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH |
| NS3–NS4A protease (genotype 5a) | Hepatitis C virus | anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH | 1.7 | 0.0066 | 260 | pH 8, 23 °C | PMID 19944069 | Open record: NS3–NS4A protease (genotype 5a), anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH |
| NS3–NS4A protease (genotype 6a) | Hepatitis C virus | anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH | 0.83 | 0.0028 | 300 | pH 8, 23 °C | PMID 19944069 | Open record: NS3–NS4A protease (genotype 6a), anthraniloyl-DDIVP-Abu[C(O)-O] AMY(3-NO_2)-TW-OH |