EC 1.5.1.15: methylenetetrahydrofolate dehydrogenase (NAD+)
OxidoreductasesOpen in Browse
CatLog read 10 records for EC 1.5.1.15 from 2 papers, for 2 organisms. Names in these papers: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 36 records for EC 1.5.1.15 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.5.1.15 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues | Rattus norvegicus | 6 |
| Human mitochondrial MTHFD2 is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase | Homo sapiens | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase) | Homo sapiens | 5,10-methylene-tetrahydropteroyl monoglutamate25 mM potassium phosphate; NAD+ 1.0 mM (saturating) | 12.4 | 0.133 | 93 | pH 8, 30 °C | PMID 29225823 | Open record: MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase), 5,10-methylene-tetrahydropteroyl monoglutamate |
| MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase) | Homo sapiens | 5,10-methylene-tetrahydropteroyl monoglutamateno phosphate; NADP+ 6.0 mM (saturating); cosubstrate NADP+ | 1.5 | 0.123 | 12 | pH 8, 30 °C | PMID 29225823 | Open record: MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase), 5,10-methylene-tetrahydropteroyl monoglutamate |
| MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase) | Homo sapiens | 5,10-methylene-tetrahydropteroyl pentaglutamate25 mM potassium phosphate; NAD+ 1.0 mM (saturating) | 15.4 | 0.359 | 43 | pH 8, 30 °C | PMID 29225823 | Open record: MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase), 5,10-methylene-tetrahydropteroyl pentaglutamate |
| MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase) | Homo sapiens | 5,10-methylene-tetrahydropteroyl pentaglutamateno phosphate; NADP+ 6.0 mM (saturating); cosubstrate NADP+ | 6.4 | 0.302 | 21 | pH 8, 30 °C | PMID 29225823 | Open record: MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase), 5,10-methylene-tetrahydropteroyl pentaglutamate |
| MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase) | Rattus norvegicus | 5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate)5 mM MgCl2; 25 mM potassium phosphate | 2.7 | 0.04 | 67 | pH 8, 30 °C | PMID 24733394 | Open record: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), 5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate) |
| MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase) | Rattus norvegicus | 5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate)5 mM MgCl2 (Methods reaction buffer); no phosphate | 1.3 | 0.042 | 30 | pH 8, 30 °C | PMID 24733394 | Open record: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), 5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate) |
| MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase) | Rattus norvegicus | 5,10-methylenetetrahydropteroylpentaglutamate5 mM MgCl2; 25 mM potassium phosphate | 8.8 | 0.13 | 68 | pH 8, 30 °C | PMID 24733394 | Open record: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), 5,10-methylenetetrahydropteroylpentaglutamate |
| MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase) | Rattus norvegicus | 5,10-methylenetetrahydropteroylpentaglutamate5 mM MgCl2 (Methods reaction buffer); no phosphate | 7.2 | 0.153 | 47 | pH 8, 30 °C | PMID 24733394 | Open record: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), 5,10-methylenetetrahydropteroylpentaglutamate |
| MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase) | Rattus norvegicus | NAD+ | 3.6 | 0.147 | 25 | pH 8, 30 °C | PMID 24733394 | Open record: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), NAD+ |
| MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase) | Rattus norvegicus | NADP+ | 1.1 | 0.537 | 2 | pH 8, 30 °C | PMID 24733394 | Open record: MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), NADP+ |