CatLogenzyme kinetics catalog

EC 1.5.1.5: methylenetetrahydrofolate dehydrogenase (NADP+)

OxidoreductasesOpen in Browse

CatLog read 28 records for EC 1.5.1.5 from 4 papers, for 4 organisms. Names in these papers: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD), MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC), C1-tetrahydrofolate synthase (methylenetetrahydrofolate dehydrogenase activity).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 56 records for EC 1.5.1.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.5.1.5 in Browse.

Papers

PaperOrganismsRecords
Impact of Mutating the Key Residues of a Bifunctional 5,10-Methylenetetrahydrofolate Dehydrogenase-Cyclohydrolase from Escherichia coli on Its ActivitiesEscherichia coli13
Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissuesRattus norvegicus6
Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologuePhotobacterium phosphoreum5
C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domainsOryctolagus cuniculus4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)Escherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate40.880.0261.58 × 10³pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)Escherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate19.920.27970pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)Escherichia coliNADP+17.080.18792pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58YEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate4.750.04120pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58YEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate2.580.3028.3pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58YEscherichia coliNADP+2.780.23312pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant D121AEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate0.010.0150.75pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant D121AEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate0.0120.1040.12pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant G122DEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate0.0250.00455.75pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant G122DEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate0.0580.0680.83pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant K54SEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate14.330.20570pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant K54SEscherichia coliNADP+12.270.14383pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant R191EEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate57.430.0282.07 × 10³pH 7.6PMID 25988590Open
C1-tetrahydrofolate synthase (methylenetetrahydrofolate dehydrogenase activity)Oryctolagus cuniculus5,10-methylenetetrahydrofolatenative enzyme purified from rabbit liver—0.002—PMID 3871768Open
C1-tetrahydrofolate synthase (methylenetetrahydrofolate dehydrogenase activity)Oryctolagus cuniculus5,10-methylenetetrahydrofolatechymotryptic fragment of the rabbit liver enzyme—0.002—PMID 3871768Open
C1-tetrahydrofolate synthase (methylenetetrahydrofolate dehydrogenase activity)Oryctolagus cuniculusNADP+native enzyme purified from rabbit liver—0.004—PMID 3871768Open
C1-tetrahydrofolate synthase (methylenetetrahydrofolate dehydrogenase activity)Oryctolagus cuniculusNADP+chymotryptic fragment of the rabbit liver enzyme—0.004—PMID 3871768Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreum(6R,S)-5,10-methenyltetrahydrofolate53—1.7 × 10³PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreum(6R,S)-5,10-methylenetetrahydrofolate—0.011—PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreumNAD+1364.7629PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreumNAD+—30.3—pH 7.3PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreumNADP+—0.2562PMID 8765228Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate)5 mM MgCl2; 25 mM potassium phosphate2.70.0467pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate)5 mM MgCl2 (Methods reaction buffer); no phosphate1.30.04230pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylpentaglutamate5 mM MgCl2; 25 mM potassium phosphate8.80.1368pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylpentaglutamate5 mM MgCl2 (Methods reaction buffer); no phosphate7.20.15347pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicusNAD+3.60.14725pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicusNADP+1.10.5372pH 8, 30 °CPMID 24733394Open