Values CatLog read from this paper
Impact of Mutating the Key Residues of a Bifunctional 5,10-Methylenetetrahydrofolate Dehydrogenase-Cyclohydrolase from Escherichia coli on Its Activities
PMID 25988590doi:10.1021/acs.biochem.5b00400Open in Browse
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CatLog read 13 records (39 values) from this paper: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) | Escherichia coli | (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate | 40.88 | 0.026 | 1.58 × 10³ | pH 7.6 | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD), (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) | Escherichia coli | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | 19.92 | 0.279 | 70 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD), (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) | Escherichia coli | NADP+ | 17.08 | 0.187 | 92 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD), NADP+ |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58Y | Escherichia coli | (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate | 4.75 | 0.04 | 120 | pH 7.6 | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant C58Y, (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58Y | Escherichia coli | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | 2.58 | 0.302 | 8.3 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant C58Y, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58Y | Escherichia coli | NADP+ | 2.78 | 0.233 | 12 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant C58Y, NADP+ |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant D121A | Escherichia coli | (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate | 0.01 | 0.015 | 0.75 | pH 7.6 | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant D121A, (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant D121A | Escherichia coli | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | 0.012 | 0.104 | 0.12 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant D121A, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant G122D | Escherichia coli | (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate | 0.025 | 0.0045 | 5.75 | pH 7.6 | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant G122D, (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant G122D | Escherichia coli | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | 0.058 | 0.068 | 0.83 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant G122D, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant K54S | Escherichia coli | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate | 14.33 | 0.205 | 70 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant K54S, (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant K54S | Escherichia coli | NADP+ | 12.27 | 0.143 | 83 | pH 7.6, 30 °C | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant K54S, NADP+ |
| bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant R191E | Escherichia coli | (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate | 57.43 | 0.028 | 2.07 × 10³ | pH 7.6 | Open record: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) variant R191E, (6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate |