CatLogenzyme kinetics catalog

EC 3.5.4.9: methenyltetrahydrofolate cyclohydrolase

HydrolasesOpen in Browse

CatLog read 30 records for EC 3.5.4.9 from 5 papers, for 5 organisms. Names in these papers: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD), MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase), methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC), MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase) and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 45 records for EC 3.5.4.9 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.4.9 in Browse.

Papers

PaperOrganismsRecords
Impact of Mutating the Key Residues of a Bifunctional 5,10-Methylenetetrahydrofolate Dehydrogenase-Cyclohydrolase from Escherichia coli on Its ActivitiesEscherichia coli13
Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissuesRattus norvegicus6
Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologuePhotobacterium phosphoreum5
Human mitochondrial MTHFD2 is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolaseHomo sapiens4
C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domainsOryctolagus cuniculus2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)Escherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate40.880.0261.58 × 10³pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)Escherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate19.920.27970pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)Escherichia coliNADP+17.080.18792pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58YEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate4.750.04120pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58YEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate2.580.3028.3pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant C58YEscherichia coliNADP+2.780.23312pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant D121AEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate0.010.0150.75pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant D121AEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate0.0120.1040.12pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant G122DEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate0.0250.00455.75pH 7.6PMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant G122DEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate0.0580.0680.83pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant K54SEscherichia coli(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate14.330.20570pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant K54SEscherichia coliNADP+12.270.14383pH 7.6, 30 °CPMID 25988590Open
bifunctional 5,10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD)variant R191EEscherichia coli(6R)-5,10-methenyl-5,6,7,8-tetrahydrofolate57.430.0282.07 × 10³pH 7.6PMID 25988590Open
C1-tetrahydrofolate synthase (methenyltetrahydrofolate cyclohydrolase activity)Oryctolagus cuniculus5,10-methenyltetrahydrofolatenative enzyme purified from rabbit liver—0.015—PMID 3871768Open
C1-tetrahydrofolate synthase (methenyltetrahydrofolate cyclohydrolase activity)Oryctolagus cuniculus5,10-methenyltetrahydrofolatechymotryptic fragment of the rabbit liver enzyme—0.016—PMID 3871768Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreum(6R,S)-5,10-methenyltetrahydrofolate53—1.7 × 10³PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreum(6R,S)-5,10-methylenetetrahydrofolate—0.011—PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreumNAD+1364.7629PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreumNAD+—30.3—pH 7.3PMID 8765228Open
methylenetetrahydrofolate dehydrogenase-cyclohydrolase (PPDC)Photobacterium phosphoreumNADP+—0.2562PMID 8765228Open
MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase)Homo sapiens5,10-methylene-tetrahydropteroyl monoglutamate25 mM potassium phosphate; NAD+ 1.0 mM (saturating)12.40.13393pH 8, 30 °CPMID 29225823Open
MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase)Homo sapiens5,10-methylene-tetrahydropteroyl monoglutamateno phosphate; NADP+ 6.0 mM (saturating); cosubstrate NADP+1.50.12312pH 8, 30 °CPMID 29225823Open
MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase)Homo sapiens5,10-methylene-tetrahydropteroyl pentaglutamate25 mM potassium phosphate; NAD+ 1.0 mM (saturating)15.40.35943pH 8, 30 °CPMID 29225823Open
MTHFD2 (mitochondrial bifunctional 5,10-methylene-THF dehydrogenase/5,10-methenyl-THF cyclohydrolase)Homo sapiens5,10-methylene-tetrahydropteroyl pentaglutamateno phosphate; NADP+ 6.0 mM (saturating); cosubstrate NADP+6.40.30221pH 8, 30 °CPMID 29225823Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate)5 mM MgCl2; 25 mM potassium phosphate2.70.0467pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylmonoglutamate (5,10-methylenetetrahydrofolate)5 mM MgCl2 (Methods reaction buffer); no phosphate1.30.04230pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylpentaglutamate5 mM MgCl2; 25 mM potassium phosphate8.80.1368pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicus5,10-methylenetetrahydropteroylpentaglutamate5 mM MgCl2 (Methods reaction buffer); no phosphate7.20.15347pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicusNAD+3.60.14725pH 8, 30 °CPMID 24733394Open
MTHFD2L (mitochondrial bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase)Rattus norvegicusNADP+1.10.5372pH 8, 30 °CPMID 24733394Open