CatLogenzyme kinetics catalog

EC 1.7.3.3: factor-independent urate hydroxylase

OxidoreductasesOpen in Browse

CatLog read 16 records for EC 1.7.3.3 from 4 papers, for 3 organisms. Names in these papers: uricase (urate oxidase, LM-UOX), uricase (urate oxidase), uricase, urate oxidase (uricase, AFUOX).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 59 records for EC 1.7.3.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.7.3.3 in Browse.

Papers

PaperOrganismsRecords
Production and Characterization of Recombinant Wild Type Uricase from Indonesian Coelacanth (L. menadoensis) and Improvement of Its Thermostability by In Silico Rational Design and Disulphide…Latimeria menadoensis6
Structure-based characterization of canine-human chimeric uricases and its evolutionary implicationscanine5
Biosynthesized uricase with dual-terminus zwitterionic EKylation for prolonged elimination half-life and reduced immunogenicity—3
The role of Gln269Leu mutation on the thermostability and structure of uricase from Aspergillus flavusAspergillus flavus2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
urate oxidase (uricase, AFUOX)Aspergillus flavusuric acid3.1250.08935.11pH 8.5, 25 °CPMID 40064946Open
urate oxidase (uricase, AFUOX)variant Q269LAspergillus flavusuric acid3.7250.0753.22pH 8.5, 25 °CPMID 40064946Open
uricase—uric acid0.80.011271.5pH 8.5, 37 °CPMID 41709250Open
uricasevariant Uri-3r20NC—uric acid10.550.0363290pH 8.5, 37 °CPMID 41709250Open
uricasevariant Uri-60NC—uric acid9.50.0431218pH 8.5, 37 °CPMID 41709250Open
uricase (urate oxidase)canineuric acid13.60.00851583pH 8.6, 25 °CPMID 22481018Open
uricase (urate oxidase)variant A296Vcanineuric acid16.080.00642513pH 8.6, 25 °CPMID 22481018Open
uricase (urate oxidase)variant A89T/G91A/V92M/H245L/E252A/M253I/R291K/A296V/A301S/K303R (UHC_H2)canineuric acid8.70.017512pH 8.6, 25 °CPMID 22481018Open
uricase (urate oxidase)variant H245L/E252A/M253I/R291K/A296V/A301S/K303R (UHC, HU245-304)canineuric acid15.50.00652385pH 8.6, 25 °CPMID 22481018Open
uricase (urate oxidase)variant R291K/A296V/A301S/K303R (HU291-304)canineuric acid16.780.00642622pH 8.6, 25 °CPMID 22481018Open
uricase (urate oxidase, LM-UOX)Latimeria menadoensisuric acid29.890.018431.62 × 10³pH 8, 30 °CPMID 30871218Open
uricase (urate oxidase, LM-UOX)variant A132C/A225CLatimeria menadoensisuric acid58.190.039191.49 × 10³pH 8, 30 °CPMID 30871218Open
uricase (urate oxidase, LM-UOX)variant D288CLatimeria menadoensisuric acid65.070.1897340pH 8, 30 °CPMID 30871218Open
uricase (urate oxidase, LM-UOX)variant I27C/N289CLatimeria menadoensisuric acid32.580.03539920pH 8, 30 °CPMID 30871218Open
uricase (urate oxidase, LM-UOX)variant N289CLatimeria menadoensisuric acid34.060.030461.12 × 10³pH 8, 30 °CPMID 30871218Open
uricase (urate oxidase, LM-UOX)variant S136CLatimeria menadoensisuric acid22.490.015281.47 × 10³pH 8, 30 °CPMID 30871218Open