CatLogenzyme kinetics catalog

EC 3.1.1.84: cocaine esterase

HydrolasesOpen in Browse

CatLog read 48 records for EC 3.1.1.84 from 7 papers, for 4 organisms. Names in these papers: cocaine esterase (CocE), hCE-2, cocaine esterase (cocE), butyrylcholinesterase and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 18 records for EC 3.1.1.84 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.1.84 in Browse.

Papers

PaperOrganismsRecords
Characterization of pyrethroid hydrolysis by the human liver carboxylesterases hCE-1 and hCE-2Homo sapiens14
Cell permeable cocaine esterases constructed by chemical conjugation and genetic recombinationRhidococcus sp. MB112
Biochemical characterization and structural analysis of a highly proficient cocaine esteraseRhodococcus sp.10
Structural analysis of thermostabilizing mutations of cocaine esteraseRhodococcus sp. MB15
Rational design, preparation, and characterization of a therapeutic enzyme mutant with improved stability and function for cocaine detoxification—3
Design, preparation, and characterization of high-activity mutants of human butyrylcholinesterase specific for detoxification of cocaineHomo sapiens2
Catalytic activities of cocaine hydrolases against the most toxic cocaine metabolite norcocaethyleneHomo sapiens2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
butyrylcholinesterasevariant A199S/F227A/S287G/A328W/E441DHomo sapiens(-)-cocaine28.80.00112.7 × 10⁴25 °CPMID 20971807Open
butyrylcholinesterasevariant A199S/F227A/S287G/A328W/Y332G/E441DHomo sapiens(-)-cocaine73.80.00352.2 × 10⁴25 °CPMID 20971807Open
butyrylcholinesterase (BChE)variant A199S/F227A/S287G/A328W/Y332G (E12–7)Homo sapiensnorcocaethylene3.50.0066530pH 7.4, 25 °CPMID 32101217Open
butyrylcholinesterase (BChE)variant A199S/S287G/A328W/Y332G (E14–3)Homo sapiensnorcocaethylene1.180.0057208pH 7.4, 25 °CPMID 32101217Open
cocaine esterase (CocE)variant T172R/G173Q—(-)-cocaine430.00291.53 × 10⁴37 °CPMID 24919140Open
cocaine esterase (CocE)variant T172R/G173Q/G4C/S10C—(-)-cocaine390.00211.8 × 10⁴37 °CPMID 24919140Open
cocaine esterase (CocE)variant T172R/G173Q/L196C/I301C—(-)-cocaine57.50.00153.8 × 10⁴37 °CPMID 24919140Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24044.850.02881.56 × 10³pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24039.20.03051283pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24029.920.02151395pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24032.920.02311427pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24032.620.0396823pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A240—0.049702pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24031.450.03061028pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainereal-time spectrophotometric assay; decrease in A24035.20.0415847pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainePBS pH 7.4; apparent kcat; apparent Km; apparent kcat/Km32.870.0698470pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainePBS pH 7.4; apparent kcat; apparent Km; apparent kcat/Km10.850.159268pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainePBS pH 7.4; apparent kcat; apparent Km; apparent kcat/Km11.780.1142103pH 7.4PMID 22404344Open
cocaine esterase (CocE)variant T172R/G173QRhidococcus sp. MB1cocainePBS pH 7.4; apparent kcat; apparent Km; apparent kcat/Km17.380.51833pH 7.4PMID 22404344Open
cocaine esterase (cocE)Rhodococcus sp.(-)-cocaine7.86.4 × 10⁻⁴1.2 × 10⁴pH 7.4PMID 12369817Open
cocaine esterase (cocE)Rhodococcus sp.cocaethylene9.40.00165.9 × 10³pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant F261ARhodococcus sp.(-)-cocaine0.270.009628pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant F408ARhodococcus sp.(-)-cocaine0.0570.005111pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant L407ARhodococcus sp.(-)-cocaine0.0670.001256pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant Q55ARhodococcus sp.(-)-cocaine1.77.5 × 10⁻⁴2.3 × 10³pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant Q55ERhodococcus sp.(-)-cocaine0.552.7 × 10⁻⁴2 × 10³pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant S117CRhodococcus sp.(-)-cocaine0.0460.0461pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant W151ARhodococcus sp.(-)-cocaine0.10.0512pH 7.4PMID 12369817Open
cocaine esterase (cocE)variant W166ARhodococcus sp.(-)-cocaine0.270.003675pH 7.4PMID 12369817Open
cocaine esterase (CocE)Rhodococcus sp. MB1cocaine51.40.00578.99 × 10³pH 7.4PMID 20436035Open
cocaine esterase (CocE)variant G173QRhodococcus sp. MB1cocaine36.10.00379.88 × 10³pH 7.4PMID 20436035Open
cocaine esterase (CocE)variant L169KRhodococcus sp. MB1cocaine80.10.0441.84 × 10³pH 7.4PMID 20436035Open
cocaine esterase (CocE)variant T172RRhodococcus sp. MB1cocaine50.90.0173.06 × 10³pH 7.4PMID 20436035Open
cocaine esterase (CocE)variant T172R/G173QRhodococcus sp. MB1cocaine53.40.0173.18 × 10³pH 7.4PMID 20436035Open
hCE-2Homo sapiens(2R)(αR)-A40.00859 × 10⁻⁴9.3pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(2R)(αS)-A40.0580.00230pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αR)(1R)-cis-A30.00189 × 10⁻⁴2.3pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αR)(1R)-trans-A30.00530.00252.2pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αR)(1S)-cis-A30.00170.00171pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αR)(1S)-trans-A30.0370.001920pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αS)(1R)-cis-A30.00470.00251.8pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αS)(1R)-trans-A30.0080.00282.8pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αS)(1S)-cis-A30.01180.0043pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiens(αS)(1S)-trans-A30.0770.003820pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiensA60.00570.00212.7pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiensA80.00520.00541pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapienscypermethrin0.0670.00917.3pH 8, 37 °CPMID 16359636Open
hCE-2Homo sapiensp-nitrophenyl acetate700.0312.33 × 10³pH 8, 37 °CPMID 16359636Open