CatLogenzyme kinetics catalog

EC 3.1.11.2: exodeoxyribonuclease III

HydrolasesOpen in Browse

CatLog read 16 records for EC 3.1.11.2 from 2 papers, for 3 organisms. Names in these papers: AP-endonuclease/3'-5' exonuclease III (MtbXthA), TREX1 (three prime repair exonuclease 1).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 4 records for EC 3.1.11.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.11.2 in Browse.

Papers

PaperOrganismsRecords
Critical determinants for substrate recognition and catalysis in the M. tuberculosis class II AP-endonuclease/3'-5' exonuclease IIIMycobacterium tuberculosis8
Three prime repair exonuclease 1 preferentially degrades the integration-incompetent HIV-1 DNA through favorable kinetics, thermodynamic, structural, and conformational propertiesHomo sapiens, Mus musculus8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
AP-endonuclease/3'-5' exonuclease III (MtbXthA)Mycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0121.5 × 10⁻⁵780pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant D251AMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0021.5 × 10⁻⁵130pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant E57AMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0011.6 × 10⁻⁵60pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant F242SMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0121.4 × 10⁻⁵880pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant W235SMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0121.8 × 10⁻⁵650pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant Y137SMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0086.4 × 10⁻⁵110pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant Y234SMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.0122.2 × 10⁻⁵550pH 7.8, 37 °CPMID 25748880Open
AP-endonuclease/3'-5' exonuclease III (MtbXthA)variant Y234S/W235SMycobacterium tuberculosis5'-FAM-labelled duplex DNA containing the abasic site analogue tetrahydrofuran (THF)0.011.6 × 10⁻⁵630pH 7.8, 37 °CPMID 25748880Open
TREX1 (three prime repair exonuclease 1)Homo sapiens20-mer blunt-ended double-stranded HIV-1 U3 LTR end DNA: 5'-6-fluorescein-AGTGAATTAGCCCTTCCAGT-3' annealed to the complementary 20-mer 5'-ACTGGAAGGGCTAATTCACT-3'9.81.507 × 10⁻⁴6.53 × 10⁴pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Homo sapiens20-mer blunt-ended double-stranded HIV-1 U5 LTR end DNA: 5'-6-fluorescein-TGTGGAAAATCTCTAGCAGT-3' annealed to the complementary 20-mer 5'-ACTGCTAGAGATTTTCCACA-3'10.29.85 × 10⁻⁵1.031 × 10⁵pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Homo sapiens3'-processed double-stranded HIV-1 U3 LTR end DNA with a recessed 3'-end: 18-mer 5'-6-fluorescein-AGTGAATTAGCCCTTCCA-3' (lacking the 3'-GT dinucleotide) annealed to the complementary 20-mer 5'-ACTGGAAGGGCTAATTCACT-3'4.51.604 × 10⁻⁴2.805 × 10⁴pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Homo sapiens3'-processed double-stranded HIV-1 U5 LTR end DNA with a recessed 3'-end: 18-mer 5'-6-fluorescein-TGTGGAAAATCTCTAGCA-3' (lacking the 3'-GT dinucleotide) annealed to the complementary 20-mer 5'-ACTGCTAGAGATTTTCCACA-3'3.85.42 × 10⁻⁵7.02 × 10⁴pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Mus musculus20-mer blunt-ended double-stranded HIV-1 U3 LTR end DNA: 5'-6-fluorescein-AGTGAATTAGCCCTTCCAGT-3' annealed to the complementary 20-mer 5'-ACTGGAAGGGCTAATTCACT-3'12.69.87 × 10⁻⁵1.278 × 10⁵pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Mus musculus20-mer blunt-ended double-stranded HIV-1 U5 LTR end DNA: 5'-6-fluorescein-TGTGGAAAATCTCTAGCAGT-3' annealed to the complementary 20-mer 5'-ACTGCTAGAGATTTTCCACA-3'9.51.095 × 10⁻⁴8.67 × 10⁴pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Mus musculus3'-processed double-stranded HIV-1 U3 LTR end DNA with a recessed 3'-end: 18-mer 5'-6-fluorescein-AGTGAATTAGCCCTTCCA-3' (lacking the 3'-GT dinucleotide) annealed to the complementary 20-mer 5'-ACTGGAAGGGCTAATTCACT-3'6.31.253 × 10⁻⁴5.46 × 10⁴pH 7.5, 37 °CPMID 38838778Open
TREX1 (three prime repair exonuclease 1)Mus musculus3'-processed double-stranded HIV-1 U5 LTR end DNA with a recessed 3'-end: 18-mer 5'-6-fluorescein-TGTGGAAAATCTCTAGCA-3' (lacking the 3'-GT dinucleotide) annealed to the complementary 20-mer 5'-ACTGCTAGAGATTTTCCACA-3'4.19.06 × 10⁻⁵4.52 × 10⁴pH 7.5, 37 °CPMID 38838778Open