EC 3.4.22.56: caspase-3
HydrolasesOpen in Browse
CatLog read 41 records for EC 3.4.22.56 from 4 papers, for 1 organism. Names in these papers: caspase-3, caspase-7.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 2 records for EC 3.4.22.56 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.22.56 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Fluorogenic Substrates for In Situ Monitoring of Caspase-3 Activity in Live Cells | — | 23 |
| Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis | Homo sapiens | 12 |
| Structural and kinetic analysis of caspase-3 reveals role for s5 binding site in substrate recognition | Homo sapiens | 5 |
| Substrate specificities of caspase family proteases | — | 1 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.022 | 4 × 10⁻⁴ | 57 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.018 | 0.0015 | 12 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.017 | 8 × 10⁻⁴ | 20 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.013 | 4 × 10⁻⁴ | 33 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Glu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.015 | 6 × 10⁻⁴ | 23 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Glu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.0092 | 7 × 10⁻⁴ | 13 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.0083 | 3 × 10⁻⁴ | 28 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.023 | 2 × 10⁻⁴ | 97 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Pro-L-Asp-L-Lys(N6-methyl red)-6-aminohexanoyl-[N-(6-aminohexyl)glycine]9 amide | 0.035 | 0.0011 | 8.3 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Pro-L-Asp-L-Lys(N6-methyl red)-6-aminohexanoyl-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.015 | 8 × 10⁻⁴ | 20 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.028 | 2 × 10⁻⁴ | 135 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.023 | 0.0018 | 13 | PMID 27168077 | Open record: caspase-3, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-3 | Homo sapiens | acetyl-Asp-Glu-Val-Asp-p-nitroanilide | 0.885 | 0.0671 | 13.17 | pH 7.5 | PMID 16781734 | Open record: caspase-3, acetyl-Asp-Glu-Val-Asp-p-nitroanilide |
| caspase-3 | Homo sapiens | acetyl-Asp-Met-Gln-Asp-p-nitroanilide | 3.53 | 1.600 | 2.207 | pH 7.5 | PMID 16781734 | Open record: caspase-3, acetyl-Asp-Met-Gln-Asp-p-nitroanilide |
| caspase-3 | Homo sapiens | acetyl-Asp-Val-Ala-Asp-p-nitroanilide | 0.903 | 0.2223 | 4.063 | pH 7.5 | PMID 16781734 | Open record: caspase-3, acetyl-Asp-Val-Ala-Asp-p-nitroanilide |
| caspase-3 | Homo sapiens | acetyl-Leu-Asp-Val-Ala-Asp-p-nitroanilide | 0.843 | 0.1472 | 5.73 | pH 7.5 | PMID 16781734 | Open record: caspase-3, acetyl-Leu-Asp-Val-Ala-Asp-p-nitroanilide |
| caspase-3 | Homo sapiens | acetyl-Val-Asp-Val-Ala-Asp-p-nitroanilide | 0.807 | 0.1647 | 4.898 | pH 7.5 | PMID 16781734 | Open record: caspase-3, acetyl-Val-Asp-Val-Ala-Asp-p-nitroanilide |
| caspase-3 | Homo sapiens | N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) | 6.01 | 0.0138 | 440 | pH 7.2, 37 °C | PMID 16787777 | Open record: caspase-3, N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) |
| caspase-3 | Homo sapiens | N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) | 0.33 | 0.0122 | 27 | pH 4.75, 37 °C | PMID 16787777 | Open record: caspase-3, N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) |
| caspase-3 | Homo sapiens | N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) | 7.08 | 0.0097 | 720 | pH 7.2, 37 °C | PMID 16787777 | Open record: caspase-3, N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) |
| caspase-3 | Homo sapiens | N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) | 0.16 | 0.008 | 20 | pH 4.75, 37 °C | PMID 16787777 | Open record: caspase-3, N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant D179A | Homo sapiens | N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) | 19.56 | 0.0335 | 580 | pH 7.2, 37 °C | PMID 16787777 | Open record: caspase-3 variant D179A, N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant D179A | Homo sapiens | N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) | 1.37 | 0.0163 | 83 | pH 4.75, 37 °C | PMID 16787777 | Open record: caspase-3 variant D179A, N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant D179A | Homo sapiens | N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) | 19.04 | 0.0255 | 740 | pH 7.2, 37 °C | PMID 16787777 | Open record: caspase-3 variant D179A, N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant D179A | Homo sapiens | N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) | 1.1 | 0.013 | 84 | pH 4.75, 37 °C | PMID 16787777 | Open record: caspase-3 variant D179A, N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant Δ176-181 | Homo sapiens | N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) | 1.54 | 0.0088 | 180 | pH 7.2, 37 °C | PMID 16787777 | Open record: caspase-3 variant Δ176-181, N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant Δ176-181 | Homo sapiens | N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) | 0.08 | 0.0048 | 16 | pH 4.75, 37 °C | PMID 16787777 | Open record: caspase-3 variant Δ176-181, N-acetyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Ac-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant Δ176-181 | Homo sapiens | N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) | 1.43 | 0.0069 | 210 | pH 7.2, 37 °C | PMID 16787777 | Open record: caspase-3 variant Δ176-181, N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) |
| caspase-3variant Δ176-181 | Homo sapiens | N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) | 0.06 | 0.002 | 33 | pH 4.75, 37 °C | PMID 16787777 | Open record: caspase-3 variant Δ176-181, N-benzyloxycarbonyl-L-aspartyl-L-glutamyl-L-valyl-L-aspartic acid 7-amido-4-methylcoumarin (Z-Asp-Glu-Val-Asp-AMC) |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.027 | 0.0049 | 5 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.32 | 0.014 | 23 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Ala-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.017 | 0.0012 | 13 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.013 | 6 × 10⁻⁴ | 22 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Asn-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Glu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.018 | 5 × 10⁻⁴ | 35 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Glu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.0033 | 3 × 10⁻⁴ | 10 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.0067 | 0.0012 | 5 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Gly-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.015 | 0.0014 | 12 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.017 | 9 × 10⁻⁴ | 18 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Leu-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.023 | 0.0036 | 6.7 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Pro-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | 0.03 | 0.0041 | 6.7 | PMID 27168077 | Open record: caspase-7, 5(6)-carboxyfluorescein-6-aminohexanoyl-L-Asp-L-Val-L-Val-L-Asp-6-aminohexanoyl-L-Lys(N6-methyl red)-[N-(6-aminohexyl)glycine]9 amide | |
| caspase-7 | — | acetyl-Asp-Glu-Val-Asp aminomethylcoumarin | — | 0.011 | — | pH 7.5 | PMID 9092497 | Open record: caspase-7, acetyl-Asp-Glu-Val-Asp aminomethylcoumarin |