EC 5.4.99.25: tRNA pseudouridine55 synthase

IsomerasesOpen in Browse

CatLog read 10 records for EC 5.4.99.25 from 2 papers, for 1 organism. Names in these papers: TruB, TruB pseudouridine synthase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 3 records for EC 5.4.99.25 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 5.4.99.25 in Browse.

Papers

Papers with records for EC 5.4.99.25
Paper	Organisms	Records
Role of cysteine residues in pseudouridine synthases of different families	Escherichia coli	5
Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues	Escherichia coli	5

Records

Records for EC 5.4.99.25 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
TruB	Escherichia coli	in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine	0.12	1.46 × 10⁻⁴	750	pH 7.5, 37 °C	PMID 10529181	Open record: TruB, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine
TruBvariant C174A	Escherichia coli	in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine	0.16	1.27 × 10⁻⁴	1.3 × 10³	pH 7.5, 37 °C	PMID 10529181	Open record: TruB variant C174A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine
TruBvariant C193V	Escherichia coli	in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine	0.18	2.18 × 10⁻⁴	830	pH 7.5, 37 °C	PMID 10529181	Open record: TruB variant C193V, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine
TruBvariant C58A	Escherichia coli	in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine	0.26	1.77 × 10⁻⁴	1.5 × 10³	pH 7.5, 37 °C	PMID 10529181	Open record: TruB variant C58A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine
TruBvariant C58A/C174A/C193A (triple)	Escherichia coli	in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine	0.11	1.24 × 10⁻⁴	890	pH 7.5, 37 °C	PMID 10529181	Open record: TruB variant C58A/C174A/C193A (triple), in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine
TruB pseudouridine synthase	Escherichia coli	tRNAPhe	0.11	1.48 × 10⁻⁴	740	37 °C	PMID 10924141	Open record: TruB pseudouridine synthase, tRNAPhe
TruB pseudouridine synthasevariant K19M	Escherichia coli	tRNAPhe	0.012	0.00161	7.5	37 °C	PMID 10924141	Open record: TruB pseudouridine synthase variant K19M, tRNAPhe
TruB pseudouridine synthasevariant K19R	Escherichia coli	tRNAPhe	0.011	9 × 10⁻⁴	12	37 °C	PMID 10924141	Open record: TruB pseudouridine synthase variant K19R, tRNAPhe
TruB pseudouridine synthasevariant P20G	Escherichia coli	tRNAPhe	0.23	3.24 × 10⁻⁴	710	37 °C	PMID 10924141	Open record: TruB pseudouridine synthase variant P20G, tRNAPhe
TruB pseudouridine synthasevariant P20L	Escherichia coli	tRNAPhe	0.52	5.17 × 10⁻⁴	1 × 10³	37 °C	PMID 10924141	Open record: TruB pseudouridine synthase variant P20L, tRNAPhe