Values CatLog read from this paper
Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues
PMID 10924141doi:10.1021/bi001079nOpen in Browse
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CatLog read 7 records (21 values) from this paper: TruB pseudouridine synthase, RluA pseudouridine synthase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RluA pseudouridine synthasevariant P29G | Escherichia coli | tRNAPhe | 0.061 | 3.18 × 10⁻⁴ | 190 | 37 °C | Open record: RluA pseudouridine synthase variant P29G, tRNAPhe |
| RluA pseudouridine synthasevariant P29L | Escherichia coli | tRNAPhe | 0.051 | 4.55 × 10⁻⁴ | 11 | 37 °C | Open record: RluA pseudouridine synthase variant P29L, tRNAPhe |
| TruB pseudouridine synthase | Escherichia coli | tRNAPhe | 0.11 | 1.48 × 10⁻⁴ | 740 | 37 °C | Open record: TruB pseudouridine synthase, tRNAPhe |
| TruB pseudouridine synthasevariant K19M | Escherichia coli | tRNAPhe | 0.012 | 0.00161 | 7.5 | 37 °C | Open record: TruB pseudouridine synthase variant K19M, tRNAPhe |
| TruB pseudouridine synthasevariant K19R | Escherichia coli | tRNAPhe | 0.011 | 9 × 10⁻⁴ | 12 | 37 °C | Open record: TruB pseudouridine synthase variant K19R, tRNAPhe |
| TruB pseudouridine synthasevariant P20G | Escherichia coli | tRNAPhe | 0.23 | 3.24 × 10⁻⁴ | 710 | 37 °C | Open record: TruB pseudouridine synthase variant P20G, tRNAPhe |
| TruB pseudouridine synthasevariant P20L | Escherichia coli | tRNAPhe | 0.52 | 5.17 × 10⁻⁴ | 1 × 10³ | 37 °C | Open record: TruB pseudouridine synthase variant P20L, tRNAPhe |