EC 5.4.99.28: tRNA pseudouridine32 synthase
IsomerasesOpen in Browse
CatLog read 6 records for EC 5.4.99.28 from 2 papers, for 1 organism. Names in these papers: RluA, RluA pseudouridine synthase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 1 record for EC 5.4.99.28 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 5.4.99.28 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Role of cysteine residues in pseudouridine synthases of different families | Escherichia coli | 4 |
| Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues | Escherichia coli | 2 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| RluA | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.099 | 1.08 × 10⁻⁴ | 920 | pH 7.5, 37 °C | PMID 10529181 | Open record: RluA, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluAvariant C117A | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.07 | 4.2 × 10⁻⁴ | 170 | pH 7.5, 37 °C | PMID 10529181 | Open record: RluA variant C117A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluAvariant C117A/C128A (double) | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.074 | 1.57 × 10⁻⁴ | 470 | pH 7.5, 37 °C | PMID 10529181 | Open record: RluA variant C117A/C128A (double), in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluAvariant C128A | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.15 | 1.6 × 10⁻⁴ | 940 | pH 7.5, 37 °C | PMID 10529181 | Open record: RluA variant C128A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluA pseudouridine synthasevariant P29G | Escherichia coli | tRNAPhe | 0.061 | 3.18 × 10⁻⁴ | 190 | 37 °C | PMID 10924141 | Open record: RluA pseudouridine synthase variant P29G, tRNAPhe |
| RluA pseudouridine synthasevariant P29L | Escherichia coli | tRNAPhe | 0.051 | 4.55 × 10⁻⁴ | 11 | 37 °C | PMID 10924141 | Open record: RluA pseudouridine synthase variant P29L, tRNAPhe |