Values CatLog read from this paper
Role of cysteine residues in pseudouridine synthases of different families
PMID 10529181doi:10.1021/bi9913911Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: TruB, RluA from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RluA | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.099 | 1.08 × 10⁻⁴ | 920 | pH 7.5, 37 °C | Open record: RluA, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluAvariant C117A | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.07 | 4.2 × 10⁻⁴ | 170 | pH 7.5, 37 °C | Open record: RluA variant C117A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluAvariant C117A/C128A (double) | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.074 | 1.57 × 10⁻⁴ | 470 | pH 7.5, 37 °C | Open record: RluA variant C117A/C128A (double), in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| RluAvariant C128A | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.15 | 1.6 × 10⁻⁴ | 940 | pH 7.5, 37 °C | Open record: RluA variant C128A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| TruB | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.12 | 1.46 × 10⁻⁴ | 750 | pH 7.5, 37 °C | Open record: TruB, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| TruBvariant C174A | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.16 | 1.27 × 10⁻⁴ | 1.3 × 10³ | pH 7.5, 37 °C | Open record: TruB variant C174A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| TruBvariant C193V | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.18 | 2.18 × 10⁻⁴ | 830 | pH 7.5, 37 °C | Open record: TruB variant C193V, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| TruBvariant C58A | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.26 | 1.77 × 10⁻⁴ | 1.5 × 10³ | pH 7.5, 37 °C | Open record: TruB variant C58A, in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |
| TruBvariant C58A/C174A/C193A (triple) | Escherichia coli | in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine | 0.11 | 1.24 × 10⁻⁴ | 890 | pH 7.5, 37 °C | Open record: TruB variant C58A/C174A/C193A (triple), in vitro transcript of E. coli tRNAPhe containing [5-3H]uridine |