CatLogenzyme kinetics catalog

Values CatLog read from this paper

Site-directed mutagenesis of putative substrate-binding residues reveals a mechanism controlling the different stereospecificities of two tropinone reductases

PMID 10347221doi:10.1074/jbc.274.23.16563Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 41 records (61 values) from this paper: tropinone reductase I (TR-I), tropinone reductase II (TR-II) from Datura stramonium.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 20 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.206, EC 1.1.1.236

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
tropinone reductase I (TR-I)Datura stramoniumNADPH—0.0116—pH 7, 28 °COpen
tropinone reductase I (TR-I)Datura stramoniumtropinone25.60.117219 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant A160SDatura stramoniumNADPH—0.045—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant A160SDatura stramoniumtropinone15.10.17586.3 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant A160S/V168EDatura stramoniumNADPH—0.037—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant A160S/V168EDatura stramoniumtropinone0.17813.30.0134 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112FDatura stramoniumNADPH—0.0167—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112FDatura stramoniumtropinone5.160.510 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112YDatura stramoniumNADPH—0.0207—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112YDatura stramoniumtropinone16.40.33848.5 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112Y/A160SDatura stramoniumNADPH—0.0822—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112Y/A160SDatura stramoniumtropinone8.421.445.85 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112Y/A160S/V168EDatura stramoniumNADPH—0.0825—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112Y/A160S/V168EDatura stramoniumtropinone0.2156.010.0358 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112Y/A160S/V168E/I223L/F226LDatura stramoniumNADPH—0.0411—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant H112Y/A160S/V168E/I223L/F226LDatura stramoniumtropinone0.1353.880.0348 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant I223L/F226LDatura stramoniumNADPH—0.0132—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant I223L/F226LDatura stramoniumtropinone21.85.114.27 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant V168EDatura stramoniumNADPH—0.0396—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant V168EDatura stramoniumtropinone0.6037.40.082 calc.pH 7, 28 °COpen
tropinone reductase I (TR-I)variant V168E/H112YDatura stramoniumNADPH—0.0564—pH 7, 28 °COpen
tropinone reductase I (TR-I)variant V168E/H112YDatura stramoniumtropinone0.3897.540.0516 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)Datura stramoniumNADPH—0.0187—pH 7, 28 °COpen
tropinone reductase II (TR-II)Datura stramoniumtropinone2.730.047657.4 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant E156VDatura stramoniumNADPH—0.0201—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant E156VDatura stramoniumtropinone1.1128.70.0387 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant E156V/Y100HDatura stramoniumNADPH—0.0255—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant E156V/Y100HDatura stramoniumtropinone1.0252.50.0194 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant L210I/L213FDatura stramoniumNADPH—0.00417—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant L210I/L213FDatura stramoniumtropinone1.880.044842.0 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant S148ADatura stramoniumNADPH—0.00172—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant S148ADatura stramoniumtropinone1.770.315.7 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant S148A/E156VDatura stramoniumNADPH—0.00817—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant S148A/E156VDatura stramoniumtropinone2.526.560.384 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100HDatura stramoniumNADPH—0.0112—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100HDatura stramoniumtropinone6.844.561.50 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100H/S148ADatura stramoniumNADPH—0.00405—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100H/S148ADatura stramoniumtropinone4.5819.60.234 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100H/S148A/E156VDatura stramoniumNADPH—0.0124—pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100H/S148A/E156VDatura stramoniumtropinone4.8638.10.128 calc.pH 7, 28 °COpen
tropinone reductase II (TR-II)variant Y100H/S148A/E156V/L210I/L213FDatura stramoniumNADPH—0.0377—pH 7, 28 °COpen