CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea

PMID 10850983doi:10.1128/jb.182.13.3688-3692.2000Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 26 records (26 values) from this paper: MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase), MdhII (MJ0490-encoded malate dehydrogenase), MdhIII (M. fervidus malate dehydrogenase, MF-Mdh) from Methanococcus jannaschii, Methanothermus fervidus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.337, EC 1.1.1.375

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii(R)-Malate—0.31—pH 9.5, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii(S)-2-hydroxyglutaric acid—0.52—pH 9.5, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii(S)-Malate1 mM NAD+ (Table 2 footnote); cosubstrate NAD+—0.02—pH 9.5, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii(S)-Malate1 mM NADP+ (Table 2 footnote); cosubstrate NADP+—0.03—pH 9.5, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii(S)-Sulfolactate1 mM NAD+ (Table 2 footnote); cosubstrate NAD+—0.04—pH 9.5, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii(S)-Sulfolactate1 mM NADP+ (Table 2 footnote); cosubstrate NADP+—0.39—pH 9.5, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschii1-oxo-1,3,4,6-hexanetetracarboxylic acid (racemic; 1 part erythro-KHTCA : 2 parts threo-KHTCA)—15—pH 8, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschiiNADH—0.01—pH 8, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschiiOxalacetate0.3 mM NADH; NADH oxidation followed at 366 nm—0.13—pH 8, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschiiOxalacetate0.3 mM NADPH; NADPH oxidation followed at 366 nm—5.32—pH 8, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschiiSulfopyruvate0.3 mM NADH; NADH oxidation followed at 366 nm—0.04—pH 8, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschiiSulfopyruvate0.3 mM NADPH; NADPH oxidation followed at 366 nm—0.21—pH 8, 70 °COpen
MdhI (MJ1425-encoded 2-hydroxy acid dehydrogenase)Methanococcus jannaschiiα-Ketoglutarate—1.9—pH 8, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschii(S)-Malate1 mM NAD+ (Table 2 footnote); cosubstrate NAD+—0.15—pH 9.5, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschii(S)-Malate1 mM NADP+ (Table 2 footnote); cosubstrate NADP+—0.025—pH 9.5, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschii(S)-Sulfolactate—4.6—pH 9.5, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschiiOxalacetate0.3 mM NADH; NADH oxidation followed at 366 nm—0.25—pH 8, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschiiOxalacetate0.3 mM NADPH; NADPH oxidation followed at 366 nm—0.3—pH 8, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschiiSulfopyruvate0.3 mM NADH; NADH oxidation followed at 366 nm—1.3—pH 8, 70 °COpen
MdhII (MJ0490-encoded malate dehydrogenase)Methanococcus jannaschiiSulfopyruvate0.3 mM NADPH; NADPH oxidation followed at 366 nm—0.19—pH 8, 70 °COpen
MdhIII (M. fervidus malate dehydrogenase, MF-Mdh)Methanothermus fervidus(S)-Malate—0.05—pH 9.5, 70 °COpen
MdhIII (M. fervidus malate dehydrogenase, MF-Mdh)Methanothermus fervidus(S)-Sulfolactate—0.07—pH 9.5, 70 °COpen
MdhIII (M. fervidus malate dehydrogenase, MF-Mdh)Methanothermus fervidusOxalacetate0.3 mM NADH; NADH oxidation followed at 366 nm—0.11—pH 8, 70 °COpen
MdhIII (M. fervidus malate dehydrogenase, MF-Mdh)Methanothermus fervidusOxalacetate0.3 mM NADPH; NADPH oxidation followed at 366 nm—0.95—pH 8, 70 °COpen
MdhIII (M. fervidus malate dehydrogenase, MF-Mdh)Methanothermus fervidusSulfopyruvate0.3 mM NADH; NADH oxidation followed at 366 nm—0.07—pH 8, 70 °COpen
MdhIII (M. fervidus malate dehydrogenase, MF-Mdh)Methanothermus fervidusSulfopyruvate0.3 mM NADPH; NADPH oxidation followed at 366 nm—0.21—pH 8, 70 °COpen