Values CatLog read from this paper
Structural and mechanistic studies of Escherichia coli nitroreductase with the antibiotic nitrofurazone. Reversed binding orientations in different redox states of the enzyme
PMID 15684426doi:10.1074/jbc.m409652200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (35 values) from this paper: nitroreductase (NTR) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nitroreductase (NTR) | Escherichia coli | 2-Nitrofuranfixed NADH at 60 μM; cosubstrate NADH | 4.3 | 0.45 | 9.6 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), 2-Nitrofuran |
| nitroreductase (NTR) | Escherichia coli | 2-Nitrofuranfixed NADPH at 60 μM; cosubstrate NADPH | 4.5 | 0.47 | 9.6 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), 2-Nitrofuran |
| nitroreductase (NTR) | Escherichia coli | 2-Nitrosotoluene | — | — | 610 | pH 7, 25 °C | Open record: nitroreductase (NTR), 2-Nitrosotoluene |
| nitroreductase (NTR) | Escherichia coli | 2-Nitrotoluene | 0.21 | 1 | 0.0201 | pH 7, 25 °C | Open record: nitroreductase (NTR), 2-Nitrotoluene |
| nitroreductase (NTR) | Escherichia coli | NADHfixed nitrofurazone at 750 μM; cosubstrate nitrofurazone | 11 | 0.006 | 1.8 × 10³ calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), NADH |
| nitroreductase (NTR) | Escherichia coli | NADHfixed nitrofurantoin at 750 μM; cosubstrate nitrofurantoin | 11.5 | 0.008 | 1.4 × 10³ calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), NADH |
| nitroreductase (NTR) | Escherichia coli | NADHfixed 2-nitrofuran at 1 mM; initial rate monitored at 340 nm | 4.3 | 0.003 | 1.4 × 10³ calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), NADH |
| nitroreductase (NTR) | Escherichia coli | NADHreaction followed at 440 nm; cosubstrate nitrofurazone | — | 0.35 | — | pH 7, 25 °C | Open record: nitroreductase (NTR), NADH |
| nitroreductase (NTR) | Escherichia coli | NADPHfixed nitrofurazone at 750 μM; cosubstrate nitrofurazone | 11.3 | 0.007 | 1.6 × 10³ calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), NADPH |
| nitroreductase (NTR) | Escherichia coli | NADPHfixed nitrofurantoin at 750 μM; cosubstrate nitrofurantoin | 11.7 | 0.008 | 1.5 × 10³ calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), NADPH |
| nitroreductase (NTR) | Escherichia coli | NADPHfixed 2-nitrofuran at 1 mM; initial rate monitored at 340 nm | 4.3 | 0.003 | 1.4 × 10³ calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), NADPH |
| nitroreductase (NTR) | Escherichia coli | Nitrobenzene | — | — | 0.65 | pH 7, 25 °C | Open record: nitroreductase (NTR), Nitrobenzene |
| nitroreductase (NTR) | Escherichia coli | Nitrofurantoinfixed NADH at 60 μM; cosubstrate NADH | 11.2 | 0.19 | 59 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), Nitrofurantoin |
| nitroreductase (NTR) | Escherichia coli | Nitrofurantoinfixed NADPH at 60 μM; cosubstrate NADPH | 10.7 | 0.16 | 67 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), Nitrofurantoin |
| nitroreductase (NTR) | Escherichia coli | Nitrofurazonefixed NADH at 60 μM; cosubstrate NADH | 10.7 | 0.16 | 67 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), Nitrofurazone |
| nitroreductase (NTR) | Escherichia coli | Nitrofurazonefixed NADPH at 60 μM; cosubstrate NADPH | 10.5 | 0.17 | 62 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), Nitrofurazone |
| nitroreductase (NTR) | Escherichia coli | nitrofurazone | 225 | 1.85 | 122 calc. | pH 7, 25 °C | Open record: nitroreductase (NTR), nitrofurazone |
| nitroreductase (NTR) | Escherichia coli | Nitrosobenzene | 167 | 0.0109 | 1.52 × 10⁴ | pH 7, 25 °C | Open record: nitroreductase (NTR), Nitrosobenzene |