Values CatLog read from this paper
Reaction mechanism and structural model of ADP-forming Acetyl-CoA synthetase from the hyperthermophilic archaeon Pyrococcus furiosus: evidence for a second active site histidine residue
PMID 18372246doi:10.1074/jbc.m710218200Open in Browse
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CatLog read 28 records (28 values) from this paper: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), ADP-forming acetyl-CoA synthetase isoenzyme I α-subunit (ACD I α, AcdIa) from Pyrococcus furiosus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | acetate | — | 0.625 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), acetate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0039 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0084 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0042 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | adenosine 5'-diphosphate | — | 0.094 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | adenosine 5'-diphosphate | — | 0.093 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | adenosine 5'-triphosphate | — | 0.221 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), adenosine 5'-triphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | arsenate | — | 12 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), arsenate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | coenzyme A | — | 0.0139 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | phosphate | — | 0.272 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), phosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) | Pyrococcus furiosus | phosphate | — | 0.23 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2), phosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | acetate | — | 0.794 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, acetate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0082 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0095 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | adenosine 5'-diphosphate | — | 0.283 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | adenosine 5'-triphosphate | — | 0.473 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, adenosine 5'-triphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | coenzyme A | — | 0.041 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Eβ | Pyrococcus furiosus | phosphate | — | 0.714 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Eβ, phosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant D212Nβ | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0071 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant D212Nβ, acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant E218Dα | Pyrococcus furiosus | acetate | — | 0.783 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant E218Dα, acetate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant E218Dα | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0062 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant E218Dα, acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant E218Dα | Pyrococcus furiosus | adenosine 5'-diphosphate | — | 0.0741 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant E218Dα, adenosine 5'-diphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant E218Dα | Pyrococcus furiosus | adenosine 5'-triphosphate | — | 0.388 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant E218Dα, adenosine 5'-triphosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant E218Dα | Pyrococcus furiosus | coenzyme A | — | 0.0771 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant E218Dα, coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant E218Dα | Pyrococcus furiosus | phosphate | — | 0.919 | — | pH 6.5, 55 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant E218Dα, phosphate |
| ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2)variant H71Aβ | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0098 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I (ACD I, α2β2) variant H71Aβ, acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I α-subunit (ACD I α, AcdIa) | Pyrococcus furiosus | acetyl coenzyme A | — | 0.0037 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I α-subunit (ACD I α, AcdIa), acetyl coenzyme A |
| ADP-forming acetyl-CoA synthetase isoenzyme I α-subunit (ACD I α, AcdIa) | Pyrococcus furiosus | arsenate | — | 15.19 | — | pH 6.5, 80 °C | Open record: ADP-forming acetyl-CoA synthetase isoenzyme I α-subunit (ACD I α, AcdIa), arsenate |