CatLogenzyme kinetics catalog

Values CatLog read from this paper

Functional roles of the tetramer organization of malic enzyme

PMID 19416979doi:10.1074/jbc.m109.005082Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 80 records (90 values) from this paper: mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME), cytosolic NADP+-dependent malic enzyme (c-NADP-ME) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.38, EC 1.1.1.40

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)Homo sapiensL-malate110.90.49230 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)Homo sapiensNADP+—0.009—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant D139AHomo sapiensL-malate95.530.95100 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant D139AHomo sapiensNADP+—0.0048—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant D568AHomo sapiensL-malate136.90.97140 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant D568AHomo sapiensNADP+—0.005—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant D90AHomo sapiensL-malate94.570.68140 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant D90AHomo sapiensNADP+—0.0039—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H142AHomo sapiensL-malate122.70.89140 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H142AHomo sapiensNADP+—0.004—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H142A/D568AHomo sapiensL-malate98.030.92110 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H142A/D568AHomo sapiensNADP+—0.0042—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51AHomo sapiensL-malate109.51.1100 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51AHomo sapiensNADP+—0.0072—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51A/D139AHomo sapiensL-malate105.31.03102 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51A/D139AHomo sapiensNADP+—0.004—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51A/D90AHomo sapiensL-malate84.830.84100 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51A/D90AHomo sapiensNADP+—0.0037—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant W572AHomo sapiensL-malate106.00.98110 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant W572AHomo sapiensNADP+—0.005—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensL-malateno fumarate238.5——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensL-malate4 mM fumarate added306.4——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensNAD+no fumarate—0.68—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensNAD+4 mM fumarate added—0.32—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D139AHomo sapiensL-malateno fumarate114.5——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D139AHomo sapiensL-malate4 mM fumarate added115.4——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D139AHomo sapiensNAD+no fumarate—1.06—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D139AHomo sapiensNAD+4 mM fumarate added—0.76—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D568AHomo sapiensL-malateno fumarate124——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D568AHomo sapiensL-malate4 mM fumarate added139.6——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D568AHomo sapiensNAD+no fumarate—1.82—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant D568AHomo sapiensNAD+4 mM fumarate added—0.85—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant E90AHomo sapiensL-malateno fumarate203——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant E90AHomo sapiensL-malate4 mM fumarate added210.7——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant E90AHomo sapiensNAD+no fumarate—0.32—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant E90AHomo sapiensNAD+4 mM fumarate added—0.34—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142AHomo sapiensL-malateno fumarate117.9——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142AHomo sapiensL-malate4 mM fumarate added139.9——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142AHomo sapiensNAD+no fumarate—0.82—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142AHomo sapiensNAD+4 mM fumarate added—0.47—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142A/D568AHomo sapiensL-malateno fumarate112.8——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142A/D568AHomo sapiensL-malate4 mM fumarate added127.4——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142A/D568AHomo sapiensNAD+no fumarate—1.39—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant H142A/D568AHomo sapiensNAD+4 mM fumarate added—0.72—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P567AHomo sapiensL-malateno fumarate141.1——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P567AHomo sapiensL-malate4 mM fumarate added164.4——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P567AHomo sapiensNAD+no fumarate—0.87—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P567AHomo sapiensNAD+4 mM fumarate added—0.51—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P573AHomo sapiensL-malateno fumarate150.2——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P573AHomo sapiensL-malate4 mM fumarate added167.1——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P573AHomo sapiensNAD+no fumarate—1.2—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P573AHomo sapiensNAD+4 mM fumarate added—0.59—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P579AHomo sapiensL-malateno fumarate234.3——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P579AHomo sapiensL-malate4 mM fumarate added284.1——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P579AHomo sapiensNAD+no fumarate—0.79—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P579AHomo sapiensNAD+4 mM fumarate added—0.39—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P580AHomo sapiensL-malateno fumarate196.5——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P580AHomo sapiensL-malate4 mM fumarate added211.6——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P580AHomo sapiensNAD+no fumarate—0.95—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant P580AHomo sapiensNAD+4 mM fumarate added—0.4—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51AHomo sapiensL-malateno fumarate139.1——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51AHomo sapiensL-malate4 mM fumarate added189.9——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51AHomo sapiensNAD+no fumarate—0.99—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51AHomo sapiensNAD+4 mM fumarate added—0.51—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51A/E90AHomo sapiensL-malateno fumarate140.1——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51A/E90AHomo sapiensL-malate4 mM fumarate added143.3——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51A/E90AHomo sapiensNAD+no fumarate—0.45—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Q51A/E90AHomo sapiensNAD+4 mM fumarate added—0.43—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant V581AHomo sapiensL-malateno fumarate205.8——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant V581AHomo sapiensL-malate4 mM fumarate added234.4——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant V581AHomo sapiensNAD+no fumarate—0.83—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant V581AHomo sapiensNAD+4 mM fumarate added—0.4—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant W572AHomo sapiensL-malateno fumarate150.4——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant W572AHomo sapiensL-malate4 mM fumarate added181.9——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant W572AHomo sapiensNAD+no fumarate—1.01—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant W572AHomo sapiensNAD+4 mM fumarate added—0.54—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Y570AHomo sapiensL-malateno fumarate141.9——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Y570AHomo sapiensL-malate4 mM fumarate added165.7——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Y570AHomo sapiensNAD+no fumarate—1.07—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant Y570AHomo sapiensNAD+4 mM fumarate added—0.58—pH 7.4, 30 °COpen