CatLogenzyme kinetics catalog

Values CatLog read from this paper

Acidic residues in the purine binding site govern the 6-oxopurine specificity of the Leishmania donovani xanthine phosphoribosyltransferase

PMID 19861168doi:10.1016/j.biocel.2009.10.020Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 41 records (78 values) from this paper: xanthine phosphoribosyltransferase (LdXPRT), hypoxanthine-guanine phosphoribosyltransferase (LdHGPRT) from Leishmania donovani.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 37 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.4.2.8, EC 2.4.2.22

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
hypoxanthine-guanine phosphoribosyltransferase (LdHGPRT)variant D168ELeishmania donovaniguanine5.40.0059920 calc.pH 7.5, 25 °COpen
hypoxanthine-guanine phosphoribosyltransferase (LdHGPRT)variant D168ELeishmania donovanihypoxanthine38 × 10⁻⁴3.8 × 10³ calc.pH 7.5, 25 °COpen
hypoxanthine-guanine phosphoribosyltransferase (LdHGPRT)variant D185ELeishmania donovanihypoxanthine0.070.01494.7 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovani5-phosphoribosyl-1-pyrophosphate10 mM MgCl2; hypoxanthine fixed at 1.0 mM3.90.0288140 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovani5-phosphoribosyl-1-pyrophosphate10 mM MgCl2; xanthine fixed at 1.0 mM; cosubstrate xanthine3.90.0162240 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovani5-phosphoribosyl-1-pyrophosphate5 mM MnCl2 in place of MgCl2; xanthine fixed at 1.0 mM—0.068—pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovanihypoxanthine10 mM MgCl2; nucleobase concentration varied3.10.4586.8 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovanihypoxanthine5 mM MnCl2 in place of MgCl2; hypoxanthine varied—0.151—pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovanipyrophosphate0.02280.0390.59 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovanixanthine3.50.0073480 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)Leishmania donovaniXMP0.0280.0920.31 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovani5-phosphoribosyl-1-pyrophosphatehypoxanthine fixed at 1.0 mM; cosubstrate hypoxanthine8.40.0251330 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovani5-phosphoribosyl-1-pyrophosphatexanthine fixed at 1.0 mM; cosubstrate xanthine20.70.0382542 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovaniguanine3.3——pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovanihypoxanthine7.10.56713 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovanipyrophosphate0.00930.1330.070 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovanixanthine23.30.00783.0 × 10³ calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198DLeishmania donovaniXMP0.01030.3580.0290 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovani5-phosphoribosyl-1-pyrophosphatehypoxanthine fixed at 1.0 mM; cosubstrate hypoxanthine3.60.00341.1 × 10³ calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovani5-phosphoribosyl-1-pyrophosphatexanthine fixed at 1.0 mM; cosubstrate xanthine12.30.00731.7 × 10³ calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovaniguanine1.10.0049220 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovanihypoxanthine2.20.00211.0 × 10³ calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovaniIMP0.00620.00780.79 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovanipyrophosphateIMP fixed at 8.0 mM; cosubstrate IMP0.00450.0740.061 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovanipyrophosphateXMP fixed at 1.0 mM; cosubstrate XMP0.01180.00592.0 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovanixanthine16.40.0913180 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E198D/E215DLeishmania donovaniXMP0.01480.5330.0280 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovani5-phosphoribosyl-1-pyrophosphatehypoxanthine fixed at 1.0 mM; cosubstrate hypoxanthine6.70.0181370 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovani5-phosphoribosyl-1-pyrophosphatexanthine fixed at 1.0 mM; cosubstrate xanthine15.10.0244619 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovaniguanine1.5——pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovanihypoxanthine8.70.52417 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovanipyrophosphate0.00380.1150.033 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovanixanthine17.50.0199879 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215DLeishmania donovaniXMP0.00370.3960.0093 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovani5-phosphoribosyl-1-pyrophosphatehypoxanthine fixed at 1.0 mM; cosubstrate hypoxanthine2.10.02391 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovani5-phosphoribosyl-1-pyrophosphatexanthine fixed at 1.0 mM; cosubstrate xanthine2.60.04558 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovaniguanine0.10.0254.0 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovanihypoxanthine2.40.12919 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovanipyrophosphate0.0250.0280.89 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovanixanthine20.03557 calc.pH 7.5, 25 °COpen
xanthine phosphoribosyltransferase (LdXPRT)variant E215QLeishmania donovaniXMP0.0430.0940.46 calc.pH 7.5, 25 °COpen