CatLogenzyme kinetics catalog

Values CatLog read from this paper

Enhancement of the latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution

PMID 20735360doi:10.1042/bj20101246Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (64 values) from this paper: homoisocitrate dehydrogenase (TtHICDH), 3-isopropylmalate dehydrogenase (TtIPMDH) from Thermus thermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.85, EC 1.1.1.87

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
3-isopropylmalate dehydrogenase (TtIPMDH)Thermus thermophilus3-isopropylmalate5.70.00311.8 × 10³pH 8, 37 °COpen
3-isopropylmalate dehydrogenase (TtIPMDH)Thermus thermophilus3-isopropylmalate21——pH 8, 60 °COpen
3-isopropylmalate dehydrogenase (TtIPMDH)Thermus thermophilusNAD+—0.047120pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)Thermus thermophilus3-isopropylmalate0.0810.075pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)Thermus thermophilusNAD+—1.90.041pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScHICDHThermus thermophilus3-isopropylmalate0.42.60.16pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScHICDHThermus thermophilus3-isopropylmalate3.8——pH 8, 60 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScHICDHThermus thermophilusNAD+—1.70.23pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScM208TThermus thermophilus3-isopropylmalate0.31.40.22pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScM208TThermus thermophilusNAD+—0.410.73pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScS72IThermus thermophilus3-isopropylmalate0.32.40.11pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScS72IThermus thermophilusNAD+—1.50.17pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScV85MThermus thermophilus3-isopropylmalate0.41.40.14pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant 2ScV85MThermus thermophilusNAD+—0.920.22pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1Thermus thermophilus3-isopropylmalate2.90.594.9pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1Thermus thermophilus3-isopropylmalate24——pH 8, 60 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1Thermus thermophilusNAD+—1.22.5pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15HThermus thermophilus3-isopropylmalate5.10.95.4pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15HThermus thermophilus3-isopropylmalate46——pH 8, 60 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15HThermus thermophilusNAD+—1.24.2pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15H/M238VThermus thermophilus3-isopropylmalate1.81.90.94pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15H/M238VThermus thermophilusNAD+—1.71.1pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15H/M310RThermus thermophilus3-isopropylmalate0.61.50.4pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15H/M310RThermus thermophilusNAD+—1.60.38pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15H/V57EThermus thermophilus3-isopropylmalate1.611.6pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-1/Y15H/V57EThermus thermophilusNAD+—1.61.4pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-2/Y15H/Y217FThermus thermophilus3-isopropylmalate0.30.920.33pH 8, 37 °COpen
homoisocitrate dehydrogenase (TtHICDH)variant LR5-2/Y15H/Y217FThermus thermophilusNAD+—0.90.33pH 8, 37 °COpen