Values CatLog read from this paper
In search of sustainable chemical processes: cloning, recombinant expression, and functional characterization of the 7α- and 7β-hydroxysteroid dehydrogenases from Clostridium absonum
PMID 22198717doi:10.1007/s00253-011-3798-xOpen in Browse
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CatLog read 13 records (39 values) from this paper: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) from Clostridium absonum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | 12-Ketochenodeoxycholic acid | 2.22 × 10⁵ | 5.7 | 3.89 × 10⁴ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 12-Ketochenodeoxycholic acid |
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | 3α,12α-dihydroxy-7-oxo-5β-cholanoic acid | 1.36 × 10⁴ | 0.96 | 1.42 × 10⁴ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 3α,12α-dihydroxy-7-oxo-5β-cholanoic acid |
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid | 3.43 × 10⁵ | 1.06 | 3.24 × 10⁵ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid |
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | 3α,7α-dihydroxy-5β-cholan-24-oic acid | 9.39 × 10³ | 0.09 | 1.04 × 10⁵ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 3α,7α-dihydroxy-5β-cholan-24-oic acid |
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | 3α-Hydroxy-7,12-dioxo-5β-cholanoic acid | 3.06 × 10⁴ | 1.14 | 2.68 × 10⁴ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 3α-Hydroxy-7,12-dioxo-5β-cholanoic acid |
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | 7-ketolithocholic acid | 977 | 0.13 | 7.52 × 10³ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), 7-ketolithocholic acid |
| 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH) | Clostridium absonum | Dehydrocholic acid | 2.26 × 10⁴ | 3.5 | 6.47 × 10³ | 20 °C | Open record: 7α-hydroxysteroid dehydrogenase (Ca7α-HSDH), Dehydrocholic acid |
| 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) | Clostridium absonum | 3α,12α-dihydroxy-7-oxo-5β-cholanoic acid | 2.03 × 10⁵ | 2.3 | 8.81 × 10⁴ | 20 °C | Open record: 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH), 3α,12α-dihydroxy-7-oxo-5β-cholanoic acid |
| 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) | Clostridium absonum | 3α,7β-dihydroxy-5β-cholan-24-oic acid | 4.3 × 10⁵ | 3.06 | 1.41 × 10⁵ | 20 °C | Open record: 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH), 3α,7β-dihydroxy-5β-cholan-24-oic acid |
| 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) | Clostridium absonum | 3α-Hydroxy-7,12-dioxo-5β-cholanoic acid | 4.13 × 10⁵ | 1.5 | 2.75 × 10⁵ | 20 °C | Open record: 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH), 3α-Hydroxy-7,12-dioxo-5β-cholanoic acid |
| 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) | Clostridium absonum | 7-Ketolithocholic acid | 6.39 × 10⁵ | 2.65 | 2.41 × 10⁵ | 20 °C | Open record: 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH), 7-Ketolithocholic acid |
| 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) | Clostridium absonum | Dehydrocholic acid | 1.46 × 10⁵ | 1.58 | 9.24 × 10⁴ | 20 °C | Open record: 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH), Dehydrocholic acid |
| 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH) | Clostridium absonum | Ursocholic acid | 4.07 × 10⁵ | 2.59 | 1.57 × 10⁵ | 20 °C | Open record: 7β-hydroxysteroid dehydrogenase (Ca7β-HSDH), Ursocholic acid |