CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structural characteristics of the nonallosteric human cytosolic malic enzyme

PMID 24998673doi:10.1016/j.bbapap.2014.06.019Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 60 records (86 values) from this paper: cytosolic NADP+-dependent malic enzyme (c-NADP-ME), mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 26 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.38, EC 1.1.1.40

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)Homo sapiensL-malatewithout fumarate301.225 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)Homo sapiensL-malatewith 5 mM fumarate301.225 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)Homo sapiensNADP+without fumarate—0.0019—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)Homo sapiensNADP+with 5 mM fumarate—0.0022—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant E73KHomo sapiensL-malatewithout fumarate301.323 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant E73KHomo sapiensL-malatewith 5 mM fumarate301.520 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant E73KHomo sapiensNADP+without fumarate—0.0125—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant E73KHomo sapiensNADP+with 5 mM fumarate—0.0119—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51Q/S57K/F58I/N59E/S60T/E62D/V65A/V68F/V69H/K70R/F72L/E73K/H74K/L75M/N76T/D78P/F79L/D80E/R81K/L83I/L84Y/L85I/D87G/L88I/D90E/V99I/T101Q/S102DHomo sapiensL-malatewithout fumarate360.845 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51Q/S57K/F58I/N59E/S60T/E62D/V65A/V68F/V69H/K70R/F72L/E73K/H74K/L75M/N76T/D78P/F79L/D80E/R81K/L83I/L84Y/L85I/D87G/L88I/D90E/V99I/T101Q/S102DHomo sapiensL-malatewith 5 mM fumarate36136 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51Q/S57K/F58I/N59E/S60T/E62D/V65A/V68F/V69H/K70R/F72L/E73K/H74K/L75M/N76T/D78P/F79L/D80E/R81K/L83I/L84Y/L85I/D87G/L88I/D90E/V99I/T101Q/S102DHomo sapiensNADP+without fumarate—0.0027—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant H51Q/S57K/F58I/N59E/S60T/E62D/V65A/V68F/V69H/K70R/F72L/E73K/H74K/L75M/N76T/D78P/F79L/D80E/R81K/L83I/L84Y/L85I/D87G/L88I/D90E/V99I/T101Q/S102DHomo sapiensNADP+with 5 mM fumarate—0.0033—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59EHomo sapiensL-malatewithout fumarate270.930 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59EHomo sapiensL-malatewith 5 mM fumarate28128 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59EHomo sapiensNADP+without fumarate—0.0089—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59EHomo sapiensNADP+with 5 mM fumarate—0.0091—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73KHomo sapiensL-malatewithout fumarate281.223 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73KHomo sapiensL-malatewith 5 mM fumarate271.321 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73KHomo sapiensNADP+without fumarate—0.0046—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73KHomo sapiensNADP+with 5 mM fumarate—0.003—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73K/S102DHomo sapiensL-malatewithout fumarate281.420 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73K/S102DHomo sapiensL-malatewith 5 mM fumarate291.915 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73K/S102DHomo sapiensNADP+without fumarate—0.0045—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant N59E/E73K/S102DHomo sapiensNADP+with 5 mM fumarate—0.0067—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S102DHomo sapiensL-malatewithout fumarate291.618 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S102DHomo sapiensL-malatewith 5 mM fumarate271.914 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S102DHomo sapiensNADP+without fumarate—0.0049—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S102DHomo sapiensNADP+with 5 mM fumarate—0.0058—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57KHomo sapiensL-malatewithout fumarate321.719 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57KHomo sapiensL-malatewith 5 mM fumarate291.717 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57KHomo sapiensNADP+without fumarate—0.0045—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57KHomo sapiensNADP+with 5 mM fumarate—0.0056—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73KHomo sapiensL-malatewithout fumarate30130 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73KHomo sapiensL-malatewith 5 mM fumarate311.226 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73KHomo sapiensNADP+without fumarate—0.0069—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73KHomo sapiensNADP+with 5 mM fumarate—0.0054—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102DHomo sapiensL-malatewithout fumarate311.226 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102DHomo sapiensL-malatewith 5 mM fumarate311.324 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102DHomo sapiensNADP+without fumarate—0.0031—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102DHomo sapiensNADP+with 5 mM fumarate—0.003—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87GHomo sapiensL-malatewithout fumarate220.828 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87GHomo sapiensL-malatewith 5 mM fumarate211.119 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87GHomo sapiensNADP+without fumarate—0.0019—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87GHomo sapiensNADP+with 5 mM fumarate—0.0021—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125HHomo sapiensL-malatewithout fumarate21121 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125HHomo sapiensL-malatewith 5 mM fumarate211.316 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125HHomo sapiensNADP+without fumarate—0.0018—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125HHomo sapiensNADP+with 5 mM fumarate—0.0032—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125HHomo sapiensL-malatewithout fumarate230.829 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125HHomo sapiensL-malatewith 5 mM fumarate241.318 calc.pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125HHomo sapiensNADP+without fumarate—0.0023—pH 7.4, 30 °COpen
cytosolic NADP+-dependent malic enzyme (c-NADP-ME)variant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125HHomo sapiensNADP+with 5 mM fumarate—0.0025—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensL-malatewithout fumarate43——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensL-malatewith 5 mM fumarate55——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensNAD+without fumarate—1.4—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)Homo sapiensNAD+with 5 mM fumarate—0.3—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant K57S/E59N/K73E/D102SHomo sapiensL-malatewithout fumarate5——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant K57S/E59N/K73E/D102SHomo sapiensL-malatewith 5 mM fumarate5——pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant K57S/E59N/K73E/D102SHomo sapiensNAD+without fumarate—0.3—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME)variant K57S/E59N/K73E/D102SHomo sapiensNAD+with 5 mM fumarate—0.3—pH 7.4, 30 °COpen