Values CatLog read from this paper
Saccharomyces cerevisiae Forms D-2-Hydroxyglutarate and Couples Its Degradation to D-Lactate Formation via a Cytosolic Transhydrogenase
PMID 26774271doi:10.1074/jbc.m115.704494Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (29 values) from this paper: Dld3, Dld2, Ser3 and 1 more from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 1.1.1.95, EC 1.1.1.399, EC 1.1.2.4, EC 1.1.99.40
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Dld2 | Saccharomyces cerevisiae | D-2-hydroxyglutarateDCIP reduction assay at 600 nm: 50 mM Tris-HCl; 40 µM DCIP | 0.18 | 0.028 | 7 | pH 8, 30 °C | Open record: Dld2, D-2-hydroxyglutarate |
| Dld2 | Saccharomyces cerevisiae | D-2-hydroxyglutarateD-2HG-pyruvate transhydrogenase assay: 50 mM Tris-HCl | 0.11 | 0.074 | 1.5 | pH 8, 30 °C | Open record: Dld2, D-2-hydroxyglutarate |
| Dld2 | Saccharomyces cerevisiae | D-lactate | 1.31 | 5.215 | 0.25 | pH 8, 30 °C | Open record: Dld2, D-lactate |
| Dld3 | Saccharomyces cerevisiae | D-2-hydroxyglutarateDCIP reduction assay at 600 nm: 50 mM Tris-HCl; 40 µM DCIP | 6.6 | 0.13 | 50 | pH 8, 30 °C | Open record: Dld3, D-2-hydroxyglutarate |
| Dld3 | Saccharomyces cerevisiae | D-2-hydroxyglutarateD-2HG-pyruvate transhydrogenase assay: 50 mM Tris-HCl | 4 | 0.111 | 36 | pH 8, 30 °C | Open record: Dld3, D-2-hydroxyglutarate |
| Dld3 | Saccharomyces cerevisiae | D-lactate | 16.5 | 0.533 | 32 | pH 8, 30 °C | Open record: Dld3, D-lactate |
| Dld3 | Saccharomyces cerevisiae | pyruvate | 4.9 | 0.45 | 12 | pH 8, 30 °C | Open record: Dld3, pyruvate |
| Ser3 | Saccharomyces cerevisiae | 2-oxoglutarate | 0.2 | 0.049 | 4.3 | pH 7.4, 30 °C | Open record: Ser3, 2-oxoglutarate |
| Ser3 | Saccharomyces cerevisiae | 3-phosphoglycerate | — | 0.001 | — | pH 8, 30 °C | Open record: Ser3, 3-phosphoglycerate |
| Ser33 | Saccharomyces cerevisiae | 2-oxoglutarate | 0.25 | 0.054 | 6.6 | pH 7.4, 30 °C | Open record: Ser33, 2-oxoglutarate |
| Ser33 | Saccharomyces cerevisiae | 3-phosphoglycerate | — | 0.004 | — | pH 8, 30 °C | Open record: Ser33, 3-phosphoglycerate |