Values CatLog read from this paper
Determinants of substrate specificity in D-3-phosphoglycerate dehydrogenase. Conversion of the M. tuberculosis enzyme from one that does not use α-ketoglutarate as a substrate to one that does
PMID 31344342doi:10.1016/j.abb.2019.07.016Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: D-3-phosphoglycerate dehydrogenase (mtPGDH), D-3-phosphoglycerate dehydrogenase (ecPGDH) from Mycobacterium tuberculosis, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-3-phosphoglycerate dehydrogenase (ecPGDH) | Escherichia coli | phosphohydroxypyruvate | 3.1 | 0.068 | 46 | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (ecPGDH), phosphohydroxypyruvate |
| D-3-phosphoglycerate dehydrogenase (ecPGDH) | Escherichia coli | α-ketoglutarate | 2.1 | 0.45 | 4.7 | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (ecPGDH), α-ketoglutarate |
| D-3-phosphoglycerate dehydrogenase (ecPGDH)variant K141R | Escherichia coli | phosphohydroxypyruvate | 9.6 | 0.081 | 120 | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (ecPGDH) variant K141R, phosphohydroxypyruvate |
| D-3-phosphoglycerate dehydrogenase (ecPGDH)variant K141R | Escherichia coli | α-ketoglutarate | 0.49 | 5 | 0.098 | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (ecPGDH) variant K141R, α-ketoglutarate |
| D-3-phosphoglycerate dehydrogenase (mtPGDH) | Mycobacterium tuberculosis | phosphohydroxypyruvate | 606 | 0.153 | 4 × 10³ | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (mtPGDH), phosphohydroxypyruvate |
| D-3-phosphoglycerate dehydrogenase (mtPGDH)variant R132K | Mycobacterium tuberculosis | phosphohydroxypyruvate | 458 | 0.015 | 3.1 × 10⁴ | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (mtPGDH) variant R132K, phosphohydroxypyruvate |
| D-3-phosphoglycerate dehydrogenase (mtPGDH)variant R72A | Mycobacterium tuberculosis | α-ketoglutarate | 16.1 | 53.1 | 0.3 | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (mtPGDH) variant R72A, α-ketoglutarate |
| D-3-phosphoglycerate dehydrogenase (mtPGDH)variant R72A/R132K | Mycobacterium tuberculosis | phosphohydroxypyruvate | 490 | 0.169 | 2.9 × 10³ | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (mtPGDH) variant R72A/R132K, phosphohydroxypyruvate |
| D-3-phosphoglycerate dehydrogenase (mtPGDH)variant R72L | Mycobacterium tuberculosis | α-ketoglutarate | 4.3 | 30.6 | 0.14 | pH 7 | Open record: D-3-phosphoglycerate dehydrogenase (mtPGDH) variant R72L, α-ketoglutarate |