CatLogenzyme kinetics catalog

Values CatLog read from this paper

Dissection of the human acetylcholinesterase active center determinants of substrate specificity. Identification of residues constituting the anionic site, the hydrophobic site, and the acyl pocket

PMID 8349597doi:10.1016/s0021-9258(19)85305-xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 48 records (144 values) from this paper: human acetylcholinesterase (HuAChE), human serum butyrylcholinesterase (HuBChE) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.1.7, EC 3.1.1.8

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
human acetylcholinesterase (HuAChE)Homo sapiensacetylthiocholine6.2 × 10³0.144.4 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)Homo sapiensbutyrylthiocholine1250.3420pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)Homo sapiensp-nitrophenyl acetate1.8 × 10³5.6333pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)Homo sapienspropionylthiocholine2.7 × 10³0.251.07 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)Homo sapiensS-3,3-dimethylbutyl thioacetate8700.451.93 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)Homo sapiensS-n-propyl thioacetate1831116.7pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295AHomo sapiensacetylthiocholine6.3 × 10³0.134.87 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295AHomo sapiensbutyrylthiocholine5000.0095.55 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295AHomo sapienspropionylthiocholine1.7 × 10³0.062.78 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295LHomo sapiensacetylthiocholine2 × 10³0.21 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295LHomo sapiensbutyrylthiocholine5000.031.67 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295LHomo sapienspropionylthiocholine1.5 × 10³0.256 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295L/F297VHomo sapiensacetylthiocholine1.5 × 10³0.43.7 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295L/F297VHomo sapiensbutyrylthiocholine7000.032.33 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F295L/F297VHomo sapienspropionylthiocholine1.5 × 10³0.433.5 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F297AHomo sapiensacetylthiocholine2 × 10³0.553.7 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F297AHomo sapiensbutyrylthiocholine8300.362.3 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F297AHomo sapienspropionylthiocholine1.7 × 10³0.782.2 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F297VHomo sapiensacetylthiocholine3.7 × 10³1.23 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F297VHomo sapiensbutyrylthiocholine1 × 10³0.382.7 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F297VHomo sapienspropionylthiocholine1.7 × 10³0.72.3 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F338AHomo sapiensacetylthiocholine2.8 × 10³0.39.5 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F338AHomo sapiensbutyrylthiocholine580.867pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F338AHomo sapiensp-nitrophenyl acetate3308.638pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F338AHomo sapienspropionylthiocholine8300.342.5 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F338AHomo sapiensS-3,3-dimethylbutyl thioacetate2200.3720pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant F338AHomo sapiensS-n-propyl thioacetate8011.76.7pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W286AHomo sapiensacetylthiocholine6.7 × 10³0.41.7 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W286AHomo sapiensp-nitrophenyl acetate2.2 × 10³4.6467pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W286AHomo sapiensS-3,3-dimethylbutyl thioacetate7700.41.9 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W286AHomo sapiensS-n-propyl thioacetate1608.220pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W86AHomo sapiensacetylthiocholine1.3 × 10³93.314.3pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W86AHomo sapiensp-nitrophenyl acetate3.2 × 10³31.05 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W86AHomo sapiensS-3,3-dimethylbutyl thioacetate4700.55850pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant W86AHomo sapiensS-n-propyl thioacetate2311.52pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapiensacetylthiocholineTable I1.7 × 10³0.141.18 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapiensacetylthiocholineTable IV1.7 × 10³0.131.28 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapiensbutyrylthiocholine500.27170pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapiensp-nitrophenyl acetate1.6 × 10³5.4300pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapienspropionylthiocholine1700.171 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapiensS-3,3-dimethylbutyl thioacetate1.38 × 10³0.52.77 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337AHomo sapiensS-n-propyl thioacetate2501025pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337FHomo sapiensacetylthiocholine6.2 × 10³0.23.08 × 10⁴pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337FHomo sapiensbutyrylthiocholine1700.111.5 × 10³pH 8, 27 °COpen
human acetylcholinesterase (HuAChE)variant Y337FHomo sapienspropionylthiocholine2.3 × 10³0.151.55 × 10⁴pH 8, 27 °COpen
human serum butyrylcholinesterase (HuBChE)Homo sapiensacetylthiocholine8300.0352.38 × 10⁴pH 8, 27 °COpen
human serum butyrylcholinesterase (HuBChE)Homo sapiensbutyrylthiocholine1.83 × 10³0.053.67 × 10⁴pH 8, 27 °COpen
human serum butyrylcholinesterase (HuBChE)Homo sapienspropionylthiocholine1.7 × 10³0.0553.03 × 10⁴pH 8, 27 °COpen