CatLogenzyme kinetics catalog

Values CatLog read from this paper

Cytochrome P450 3A4-catalyzed testosterone 6beta-hydroxylation stereochemistry, kinetic deuterium isotope effects, and rate-limiting steps

PMID 15772082doi:10.1074/jbc.m501854200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (23 values) from this paper: cytochrome P450 3A4 from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.14.14.1, EC 1.14.14.55, EC 1.14.14.56, EC 1.14.14.73

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cytochrome P450 3A4Homo sapiens(2,2,4,6,6-2H5)testosteroneBaculosomes (4 pmol P450 3A4); 4 pmol P450; Table I0.88——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(2,2,4,6,6-2H5)testosteroneBaculosomes (4 pmol P450 3A4); 4 pmol P450; Table I0.033——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(2,2,4,6,6-2H5)testosteroneBaculosomes (4 pmol P450 3A4); 4 pmol P450; Table I0.237——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(2,2,4,6,6-2H5)testosteroneBaculosomes (4 pmol P450 3A4); 4 pmol P450; Table I0.227——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(2,2,4,6,6-2H5)testosterone30 pmol P450; Table II0.202——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosterone4-200 µM testosterone; 4 pmol P450; Table I0.42——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosterone4-200 µM testosterone; 4 pmol P450; Table I0.2——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosterone4-200 µM testosterone; 4 pmol P450; Table I0.107——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosterone4-200 µM testosterone; 4 pmol P450; Table I0.115——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosterone4-200 µM testosterone; 30 pmol P450; Table II0.143——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosterone4-200 µM testosterone; Table II0.115——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens(6,6-2H2)testosteroneTable II0.178——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiens7-(benzyloxy)quinoline0.05330.0143.8 calc.pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapiensd2-benzyloxyquinoline0.020.02150.93 calc.pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosterone4-200 µM testosterone; 4 pmol P450; Table I1.3——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosterone4-200 µM testosterone; 4 pmol P450; Table I0.23——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosterone4-200 µM testosterone; 4 pmol P450; Table I0.118——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosterone4-200 µM testosterone; 4 pmol P450; Table I0.118——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosterone4-200 µM testosterone; 30 pmol P450; Table II0.255——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosterone4-200 µM testosterone; Table II0.165——pH 7.4, 37 °COpen
cytochrome P450 3A4Homo sapienstestosteroneTable II0.242——pH 7.4, 37 °COpen