Values CatLog read from this paper

Construction of a synthetic metabolic pathway for biosynthesis of the non-natural methionine precursor 2,4-dihydroxybutyric acid

PMID 28631755doi:10.1038/ncomms15828Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 49 records (96 values) from this paper: aspartate semialdehyde dehydrogenase, aspartate kinase III, succinic semialdehyde reductase and 7 more from Escherichia coli, Metallosphaera sedula, Bacillus subtilis and 3 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.3, EC 1.1.1.61, EC 1.2.1.11, EC 1.2.1.76, EC 2.7.2.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
4-hydroxybutyrate dehydrogenase	Porphyromonas gingivalis	(L)-malate-beta-semialdehyde	—	5.1	0.98	pH 7.5, 37 °C	Open record: 4-hydroxybutyrate dehydrogenase, (L)-malate-beta-semialdehyde
aspartate kinase III	Escherichia coli	(L)-aspartate	13.2	7.9	1.678	pH 7.5, 37 °C	Open record: aspartate kinase III, (L)-aspartate
aspartate kinase IIIvariant E119A	Escherichia coli	(L)-malate	2.5	10.5	0.24	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119A, (L)-malate
aspartate kinase IIIvariant E119C	Escherichia coli	(L)-malate	4.4	19.2	0.23	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119C, (L)-malate
aspartate kinase IIIvariant E119G	Escherichia coli	(L)-aspartate	0.1	32.3	0.003	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119G, (L)-aspartate
aspartate kinase IIIvariant E119G	Escherichia coli	(L)-malate	1.4	10.9	0.14	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119G, (L)-malate
aspartate kinase IIIvariant E119N	Escherichia coli	(L)-malate	0.1	43	0.003	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119N, (L)-malate
aspartate kinase IIIvariant E119P	Escherichia coli	(L)-malate	0.6	18.5	0.04	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119P, (L)-malate
aspartate kinase IIIvariant E119Q	Escherichia coli	(L)-malate	0.03	38.5	0.001	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119Q, (L)-malate
aspartate kinase IIIvariant E119S	Escherichia coli	(L)-aspartate	1.1	491.7	0.002	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119S, (L)-aspartate
aspartate kinase IIIvariant E119S	Escherichia coli	(L)-malate	2.2	12.7	0.17	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119S, (L)-malate
aspartate kinase IIIvariant E119T	Escherichia coli	(L)-malate	0.4	12.3	0.03	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119T, (L)-malate
aspartate kinase IIIvariant E119V	Escherichia coli	(L)-malate	0.4	23	0.02	pH 7.5, 37 °C	Open record: aspartate kinase III variant E119V, (L)-malate
aspartate kinase IIIvariant V115A/E119S/E434V	Escherichia coli	(L)-aspartate	1.14	119.5	0.01	pH 7.5, 37 °C	Open record: aspartate kinase III variant V115A/E119S/E434V, (L)-aspartate
aspartate kinase IIIvariant V115A/E119S/E434V	Escherichia coli	(L)-malate	6.8	8.3	0.82	pH 7.5, 37 °C	Open record: aspartate kinase III variant V115A/E119S/E434V, (L)-malate
aspartate kinase III (Ec-LysC)variant V115A/E119S/E434V	Escherichia coli	L-malate	—	—	0.82	pH 7.5, 37 °C	Open record: aspartate kinase III (Ec-LysC) variant V115A/E119S/E434V, L-malate
aspartate semialdehyde dehydrogenase	Bacillus subtilis	4-phospho-(L)-aspartate	14.45	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenase	Bacillus subtilis	4-phospho-(L)-malate	0.04	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenasevariant E218C	Bacillus subtilis	4-phospho-(L)-aspartate	0.13	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E218C, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenasevariant E218C	Bacillus subtilis	4-phospho-(L)-malate	0.07	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E218C, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenasevariant E218Q	Bacillus subtilis	4-phospho-(L)-aspartate	0.15	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E218Q, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenasevariant E218Q	Bacillus subtilis	4-phospho-(L)-malate	0.31	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E218Q, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenase	Escherichia coli	4-phospho-(L)-aspartate	36.2	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenase	Escherichia coli	4-phospho-(L)-malate	0.13	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenasevariant E241C	Escherichia coli	4-phospho-(L)-aspartate	0.15	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E241C, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenasevariant E241C	Escherichia coli	4-phospho-(L)-malate	0.01	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E241C, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenasevariant E241Q	Escherichia coli	4-phospho-(L)-aspartate	0.33	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E241Q, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenasevariant E241Q	Escherichia coli	4-phospho-(L)-malate	0.08	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E241Q, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenase	Methanocaldococcus jannaschii	4-phospho-(L)-aspartate	0.38	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenase	Methanocaldococcus jannaschii	4-phospho-(L)-malate	0.04	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenasevariant E210C	Methanocaldococcus jannaschii	4-phospho-(L)-aspartate	0.09	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E210C, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenasevariant E210C	Methanocaldococcus jannaschii	4-phospho-(L)-malate	0.11	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E210C, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenasevariant E210Q	Methanocaldococcus jannaschii	4-phospho-(L)-aspartate	0.09	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E210Q, 4-phospho-(L)-aspartate
aspartate semialdehyde dehydrogenasevariant E210Q	Methanocaldococcus jannaschii	4-phospho-(L)-malate	0.08	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase variant E210Q, 4-phospho-(L)-malate
aspartate semialdehyde dehydrogenase (Bs-Asd)	Bacillus subtilis	aspartyl-4-phosphate	14.5	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase (Bs-Asd), aspartyl-4-phosphate
aspartate semialdehyde dehydrogenase (Bs-Asd)variant E218Q	Bacillus subtilis	malyl-4-phosphate	0.31	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase (Bs-Asd) variant E218Q, malyl-4-phosphate
aspartate semialdehyde dehydrogenase (Ec-Asd)	Escherichia coli	aspartyl-4-phosphate	36	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase (Ec-Asd), aspartyl-4-phosphate
aspartate semialdehyde dehydrogenase (Ec-Asd)	Escherichia coli	malyl-4-phosphate	0.13	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase (Ec-Asd), malyl-4-phosphate
aspartate semialdehyde dehydrogenase (Mj-Asd)	Methanocaldococcus jannaschii	aspartyl-4-phosphate	0.38	—	—	pH 7, 37 °C	Open record: aspartate semialdehyde dehydrogenase (Mj-Asd), aspartyl-4-phosphate
homoserine dehydrogenase	Saccharomyces cerevisiae	(L)-aspartate-beta-semialdehyde	126	0.22	573	pH 7.5, 37 °C	Open record: homoserine dehydrogenase, (L)-aspartate-beta-semialdehyde
succinic semialdehyde reductase	Metallosphaera sedula	(L)-malate-beta-semialdehyde	5.6	1.1	5	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase, (L)-malate-beta-semialdehyde
succinic semialdehyde reductase	Metallosphaera sedula	succinic semialdehyde	12.5	0.1	125	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase, succinic semialdehyde
succinic semialdehyde reductasevariant H39R	Metallosphaera sedula	(L)-malate-beta-semialdehyde	2.3	0.3	8.2	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase variant H39R, (L)-malate-beta-semialdehyde
succinic semialdehyde reductasevariant H39R	Metallosphaera sedula	succinic semialdehyde	5.1	0.05	97	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase variant H39R, succinic semialdehyde
succinic semialdehyde reductasevariant H39R/N43H	Metallosphaera sedula	(L)-malate-beta-semialdehyde	6.3	0.3	22.3	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase variant H39R/N43H, (L)-malate-beta-semialdehyde
succinic semialdehyde reductasevariant H39R/N43H	Metallosphaera sedula	succinic semialdehyde	12.3	0.05	249	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase variant H39R/N43H, succinic semialdehyde
succinic semialdehyde reductasevariant N43H	Metallosphaera sedula	(L)-malate-beta-semialdehyde	5.3	1.2	4.4	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase variant N43H, (L)-malate-beta-semialdehyde
succinic semialdehyde reductasevariant N43H	Metallosphaera sedula	succinic semialdehyde	20.4	0.14	146	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase variant N43H, succinic semialdehyde
succinic semialdehyde reductase (Ms-Ssr)	Metallosphaera sedula	(L)-malate-beta-semialdehyde	—	1.1	—	pH 7.5, 37 °C	Open record: succinic semialdehyde reductase (Ms-Ssr), (L)-malate-beta-semialdehyde