CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mouse 17alpha-hydroxysteroid dehydrogenase (AKR1C21) binds steroids differently from other aldo-keto reductases: identification and characterization of amino acid residues critical for substrate…

PMID 17442338doi:10.1016/j.jmb.2007.03.058Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 6 records (18 values) from this paper: 17α-hydroxysteroid dehydrogenase (m17α-HSD, AKR1C21), human type 3 3α-hydroxysteroid dehydrogenase (h3α-HSD3) from Mus musculus, Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.51, EC 1.1.1.62, EC 1.1.1.112, EC 1.1.1.209, EC 1.1.1.357, EC 1.3.1.20

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
17α-hydroxysteroid dehydrogenase (m17α-HSD, AKR1C21)Mus musculusandrost-4-ene-3,17-dione0.36780.0192619.1pH 7.3, 37 °COpen
17α-hydroxysteroid dehydrogenase (m17α-HSD, AKR1C21)variant A24YMus musculusandrost-4-ene-3,17-dione0.03680.12460.3pH 7.3, 37 °COpen
17α-hydroxysteroid dehydrogenase (m17α-HSD, AKR1C21)variant K31AMus musculusandrost-4-ene-3,17-dione0.77670.0227934.08pH 7.3, 37 °COpen
17α-hydroxysteroid dehydrogenase (m17α-HSD, AKR1C21)variant K31MMus musculusandrost-4-ene-3,17-dione0.66230.0227929.07pH 7.3, 37 °COpen
human type 3 3α-hydroxysteroid dehydrogenase (h3α-HSD3)variant Y24AHomo sapiensandrost-4-ene-3,17-dione17α-HSD activity (17α-reduction of Δ4)3.3 × 10⁻⁴0.020940.017pH 7.3, 37 °COpen
human type 3 3α-hydroxysteroid dehydrogenase (h3α-HSD3)variant Y24AHomo sapiensandrost-4-ene-3,17-dione17β-HSD activity (17β-reduction of Δ4 to testosterone)0.02150.029570.73pH 7.3, 37 °COpen